Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 한영복 | - |
dc.date.accessioned | 2016-08-28T11:08:02Z | - |
dc.date.available | 2016-08-28T11:08:02Z | - |
dc.date.issued | 1998 | - |
dc.identifier.issn | 0146-0404 | - |
dc.identifier.other | OAK-2 | - |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/218391 | - |
dc.description.abstract | PURPOSE. Interleukin-6 (IL-6) has been strongly implicated in anterior uveitis based on its presence in aqueous humor from infected eyes and its inflammatory effects when injected intravitreally into rats. We used IL-6- deficient mice to test further the hypothesis that IL-6 contributes to the development of endotoxin-induced uveitis. METHODS. Uveitis was scored by histologic analysis of C3H/HeN mice 24 hours after intravitreal injections of up to 200 ng of recombinant murine IL-6. Uveitis was similarly measured in IL-6-deficient mice and congenic controls 24 hours after intravitreal injection of 250 ng of Escherichia coli endotoxin. Reverse transcription- polymerase chain reaction was used to detect mRNAs for several cytokines at 3 hours postinjection. The IL-6 concentration in aqueous humor samples was determined with a bioassay using the murine B9 plasmacytoma cell line. RESULTS. Direct injection of IL-6 did not induce uveitis. Mice genetically deficient in IL-6 developed endotoxin-induced uveitis that was comparable or more severe than congenic control mice. Compensatory changes in the expression of mRNA for other cytokines were not detected in irises from the IL-6-deficient mice. In IL-6-competent mice that received bilateral endotoxin injections, no correlation was found between the number of infiltrating cells in one eye and the IL-6 concentration in the aqueous humor of the contralateral eye. CONCLUSIONS. In marked contrast to previous conclusions with rats, IL-6 was not sufficient for inducing uveitis in mice. Additionally, IL-6 was not necessary for the development of uveitis subsequent to intravitreal injection of endotoxin in mice. | - |
dc.language | English | - |
dc.title | Interleukin-6 does not mediate endotoxin-induced uveitis in mice: Studies in gene deletion animals | - |
dc.type | Article | - |
dc.relation.issue | 1 | - |
dc.relation.volume | 39 | - |
dc.relation.index | SCIE | - |
dc.relation.index | SCOPUS | - |
dc.relation.startpage | 64 | - |
dc.relation.lastpage | 69 | - |
dc.relation.journaltitle | Investigative Ophthalmology and Visual Science | - |
dc.identifier.wosid | WOS:000071410800008 | - |
dc.identifier.scopusid | 2-s2.0-0031965108 | - |
dc.author.google | Rosenbaum J.T. | - |
dc.author.google | Kievit P. | - |
dc.author.google | Han Y.B. | - |
dc.author.google | Park J.M. | - |
dc.author.google | Planck S.R. | - |
dc.date.modifydate | 20180329113948 | - |