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Crystal structure of hypothetical fructose-specific EIIB from Escherichia coli

Title
Crystal structure of hypothetical fructose-specific EIIB from Escherichia coli
Authors
Park J.Kim M.-S.Joo K.Jhon G.-J.Berry E.A.Lee J.Shin D.H.
Ewha Authors
전길자신동해
SCOPUS Author ID
전길자scopus; 신동해scopus
Issue Date
2016
Journal Title
Molecules and Cells
ISSN
1016-8478JCR Link
Citation
vol. 39, no. 6, pp. 495 - 500
Keywords
Fructose specific enzyme EIIBFrwDFunctional cysteinePTS permeasePTS systemX-ray crystallography
Publisher
Korean Society for Molecular and Cellular Biology
Indexed
SCI; SCIE; SCOPUS; KCI WOS scopus
Abstract
We have solved the crystal structure of a predicted fructose- specific enzyme IIBfruc from Escherichia coli (EcEIIBfruc) involved in the phosphoenolpyruvate-carbohydrate phosphotransferase system transferring carbohydrates across the cytoplasmic membrane. EcEIIBfruc belongs to a sequence family with more than 5,000 sequence homologues with 25-99% amino-acid sequence identity. It reveals a conventional Rossmann-like α-β-α sandwich fold with a unique β-sheet topology. Its C-terminus is longer than its closest relatives and forms an additional β-strand whereas the shorter C-terminus is random coil in the relatives. Interestingly, its core structure is similar to that of enzyme IIBcellobiose from E. coli (EcIIBcel) transferring a phosphate moiety. In the active site of the closest EcEIIBfruc homologues, a unique motif CXXGXAHT comprising a P-loop like architecture including a histidine residue is found. The conserved cysteine on this loop may be deprotonated to act as a nucleophile similar to that of EcIIBcel. The conserved histidine residue is presumed to bind the negatively charged phosphate. Therefore, we propose that the catalytic mechanism of EcEIIBfruc is similar to that of EcIIBcel transferring phosphoryl moiety to a specific carbohydrate. © The Korean Society for Molecular and Cellular Biology. All rights reserved.
DOI
10.14348/molcells.2016.0055
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자연과학대학 > 화학·나노과학전공 > Journal papers
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