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3 '-UTR engineering to improve soluble expression and fine-tuning of activity of cascade enzymes in Escherichia coli

Title
3 '-UTR engineering to improve soluble expression and fine-tuning of activity of cascade enzymes in Escherichia coli
Authors
Song, Ji-WonWoo, Ji-MinJung, Gyoo YeolBornscheuer, Uwe T.Park, Jin-Byung
Ewha Authors
박진병
SCOPUS Author ID
박진병scopus
Issue Date
2016
Journal Title
SCIENTIFIC REPORTS
ISSN
2045-2322JCR Link
Citation
vol. 6
Publisher
NATURE PUBLISHING GROUP
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
3'-Untranslated region (3'UTR) engineering was investigated to improve solubility of heterologous proteins (e.g., Baeyer-Villiger monooxygenases (BVMOs)) in Escherichia coli. Insertion of gene fragments containing putative RNase E recognition sites into the 3'UTR of the BVMO genes led to the reduction of mRNA levels in E. coli. Importantly, the amounts of soluble BVMOs were remarkably enhanced resulting in a proportional increase of in vivo catalytic activities. Notably, this increase in biocatalytic activity correlated to the number of putative RNase E endonucleolytic cleavage sites in the 3'UTR. For instance, the biotransformation activity of the BVMO BmoF1 (from Pseudomonas fluorescens DSM50106) in E. coli was linear to the number of RNase E cleavage sites in the 3'UTR. In summary, 3'UTR engineering can be used to improve the soluble expression of heterologous enzymes, thereby fine-tuning the enzyme activity in microbial cells.
DOI
10.1038/srep29406
Appears in Collections:
엘텍공과대학 > 식품공학전공 > Journal papers
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