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House Dust Mite-Derived Chitin Enhances Th2 Cell Response to Inhaled Allergens, Mainly via a TNF-alpha-Dependent Pathway

Title
House Dust Mite-Derived Chitin Enhances Th2 Cell Response to Inhaled Allergens, Mainly via a TNF-alpha-Dependent Pathway
Authors
Choi, Jun-PyoLee, Sang-MinChoi, Hyun-IlKim, Min-HyeJeon, Seong GyuJang, Myoung HoJee, Young-KooYang, SanghwaCho, Young-JooKim, Yoon-Keun
Ewha Authors
조영주김윤근최준표양상화김민혜
SCOPUS Author ID
조영주scopus; 김윤근scopusscopus; 김민혜scopus
Issue Date
2016
Journal Title
ALLERGY ASTHMA & IMMUNOLOGY RESEARCH
ISSN
2092-7355JCR Link

2092-7363JCR Link
Citation
ALLERGY ASTHMA & IMMUNOLOGY RESEARCH vol. 8, no. 4, pp. 362 - 374
Keywords
Chitin; house dust mite; Th2 cell response; TNF-alpha
Publisher
KOREAN ACAD ASTHMA ALLERGY &

CLINICAL IMMUNOLOGY
Indexed
SCIE; SCOPUS; KCI WOS scopus
Document Type
Article
Abstract
Purpose: Chitin is a potent adjuvant in the development of immune response to inhaled allergens in the airways. According to other studies, chitin is known as multi-faced adjuvants which can induce Th2 responses. Recently, we found that TNF-alpha is a key mediator in the development of Th2 cell response to inhaled allergens. Here, we evaluated the immunologic mechanisms in the development of airway hypersensitivity to inhaled allergens, enhanced by house dust mite (HDM)-derived chitin. Methods: The role of TNF-alpha and TLRs was evaluated in an airway hypersensitivity mouse model induced by a sensitization with an allergen (ovalbumin, OVA) and HDM-derived chitin using mice with the null mutation of target genes. Results: The present study showed that airway sensitization with HDM-derived chitin plus OVA enhanced OVA-induced airway inflammation v. OVA alone. This phenotype was associated with the increased expression of Th1, Th2, and Th17 cytokines and also with the enhanced production of OVA-specific IgE, IgG1, and IgG2a. As for T cell responses, OVA-specific Th2 cell response, enhanced by chitin, was abolished by the treatment of chitinase, whereas Th1 and Th17 cell responses enhanced. by this treatment. Moreover, the null mutation of the TNF-alpha gene revealed similar effects as the chitinase treatment. In contrast, all the OVA-specific T cell responses, enhanced by chitin, were blocked by the absence of TLR2, but not of TLR1, TLR4, or TLR6. Conclusions: In conclusion, these data suggest that HDM-derived chitin may enhance airway hypersensitivity to inhaled allergens, via the TLR2-dependent pathway, and that chitin-induced TNF-alpha can be a key mediator in the development of Th2 cell response to inhaled allergens.
DOI
10.4168/aair.2016.8.4.362
Appears in Collections:
의과대학 > 의학과 > Journal papers
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