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A lectin-based approach to detecting carcinogenesis in breast tissue

Title
A lectin-based approach to detecting carcinogenesis in breast tissue
Authors
Wi, Ga RamMoon, Byung-InKim, Hyoung JinLim, WoosungLee, AnbokLee, Jun WooKim, Hong-Jin
Ewha Authors
문병인임우성이안복이준우
SCOPUS Author ID
문병인scopus; 임우성scopus; 이준우scopus
Issue Date
2016
Journal Title
ONCOLOGY LETTERS
ISSN
1792-1074JCR Link1792-1082JCR Link
Citation
vol. 11, no. 6, pp. 3889 - 3895
Keywords
breast cancerglycosylationlectinenzyme-linked lectin assaymannosylationgalactosylationsialylationfucosylation
Publisher
SPANDIDOS PUBL LTD
Indexed
SCIE; SCOPUS WOS scopus
Abstract
It has been suggested that the diversity of glycosylation structures that form during cancer progression and the sensitivity with which they are able to be detected have great potential for cancer screening. However, the large majority of breast cancer research has instead focused on the development of protein or nucleic acid markers. In the present study, alterations in glycosylation in breast cancer tissue were analyzed using enzyme-linked lectin assays (ELLAs), which have potential for high-throughput screening. Cancer tissues (CCs) and normal tissues (CNs) were collected from women with breast cancer ranging from stage 0 to IIIA. The specimens were divided into two groups, stage 0-I and stage II-III, and the levels of four types of lectin in stage 0-I and stage II-III CCs and CNs were compared by ELLA. The results demonstrated that, relative to CNs, the CCs contained significantly enhanced levels of mannosylation (stage 0-I, P<0.001; stage II-III, P<0.001), galactosylation (stage 0-I, P<0.05; stage II-III, P<0.001), sialylation (stage 0-I, P<0.001; stage II-III, P<0.01) and fucosylation (stage 0-I, P<0.01; stage II-III, P<0.01). Furthermore, stage II-III CCs had higher levels of mannosylation (P<0.05) and galactosylation (P<0.01) than stage 0-I CCs. The sensitivity of the ELLA system ranged from 71-100% when specificity was set at 100%. These results demonstrate that enhanced glycosylation levels identified by ELLA are associated with the development of breast tumors, and provide evidence of the exceptional sensitivity and specificity of the ELLA system in the detection of breast cancer. This approach is anticipated to contribute highly to the development of reliable diagnostic procedures for breast cancer.
DOI
10.3892/ol.2016.4456
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의학전문대학원 > 의학과 > Journal papers
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