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dc.contributor.author이수영-
dc.date.accessioned2016-08-28T11:08:43Z-
dc.date.available2016-08-28T11:08:43Z-
dc.date.issued2016-
dc.identifier.issn0142-9612-
dc.identifier.issn1878-5905-
dc.identifier.otherOAK-18726-
dc.identifier.urihttp://dspace.ewha.ac.kr/handle/2015.oak/218253-
dc.description.abstractSmall molecule-inhibition targeting protein-protein interaction (PPI) is now recognized as an emerging and challenging area in drug design. We developed a novel interactive drug discovery methodology known as Protein Chip technology (ProteoChip) as a cutting-edge PPI assay system applicable for unique PPI-targeting therapeutics integrated with computer-aided drug design (CADD). Here, we describe a novel small molecular PPI inhibitor, IPS-02001, which the blocks integrin alpha(v)beta(3)-osteopontin interface a novel PPI inhibitor identified by the interactive methodology of both ProteoChip- and CADD-based PPI assay. IPS-02001 (6,7-Dichloro-2,3,5,8-tetrahydroxy-1,4-naphthoquinone) was screened from different compound libraries (InterBioScreen, Commercial libraries) using an in silica structure-based molecular docking simulation method and a protein chip-based protein-protein interaction assay system. Additionally, integrin alpha(v)beta(3), an adhesion receptor expressed in osteoclasts (OCs), was implicated in the regulation of OC function via regulation of the cytoskeletal organization of OCs. IPS-02001 blocked OC maturation from murine bone marrow-derived macrophages, as well as the resorptive function of OCs. Moreover, treatment with IPS-02001 impaired downstream signaling of integrin alpha(v)beta(3) linked to Pyk2, c-Src, PLC gamma 2, and Vav3 and disrupted the actin cytoskeleton in mature OCs. Furthermore, IPS-02001 blocked RANKL-induced bone destruction by reducing the number of OCs and protected against ovariectomy-induced bone loss in mice. Thus, IPS-02001 may represent a promising new class of anti-resorptive drugs for treatment of bone diseases associated with increased OC function. (C) 2016 Elsevier Ltd. All rights reserved.-
dc.languageEnglish-
dc.publisherELSEVIER SCI LTD-
dc.subjectSmall-molecule PPI inhibitor-
dc.subjectProteoChip-
dc.subjectIn silico molecular docking simulation-
dc.subjectOsteoclast inhibition-
dc.subjectBone resorption-
dc.subjectIntegrin alpha(v)beta(3)-OPN interface-
dc.titleA novel small-molecule PPI inhibitor targeting integrin alpha(v)beta(3)-osteopontin interface blocks bone resorption in vitro and prevents bone loss in mice-
dc.typeArticle-
dc.relation.volume98-
dc.relation.indexSCI-
dc.relation.indexSCIE-
dc.relation.indexSCOPUS-
dc.relation.startpage131-
dc.relation.lastpage142-
dc.relation.journaltitleBIOMATERIALS-
dc.identifier.doi10.1016/j.biomaterials.2016.05.007-
dc.identifier.wosidWOS:000379278400012-
dc.identifier.scopusid2-s2.0-84966697301-
dc.author.googlePark, Doori-
dc.author.googlePark, Chan-Won-
dc.author.googleChoi, YoungJin-
dc.author.googleLin, Jingjing-
dc.author.googleSeo, Dong-Hyun-
dc.author.googleKim, Han-Sung-
dc.author.googleLee, Soo Young-
dc.author.googleKang, In-Cheol-
dc.contributor.scopusid이수영(7409697278)-
dc.date.modifydate20190901081003-
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자연과학대학 > 생명과학전공 > Journal papers
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