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Heparin-immobilized hydroxyapatite nanoparticles as a lactoferrin delivery system for improving osteogenic differentiation of adipose-derived stem cells

Title
Heparin-immobilized hydroxyapatite nanoparticles as a lactoferrin delivery system for improving osteogenic differentiation of adipose-derived stem cells
Authors
Kim, Sung EunLee, Deok-WonYun, Young-PilShim, Kyu-SikJeon, Daniel I.Rhee, Jin-KyuKim, Hak-JunPark, Kyeongsoon
Ewha Authors
이진규
SCOPUS Author ID
이진규scopus
Issue Date
2016
Journal Title
BIOMEDICAL MATERIALS
ISSN
1748-6041JCR Link1748-605XJCR Link
Citation
vol. 11, no. 2
Keywords
hydroxyapatite nanoparticlesheparinlactoferrin deliveryadipose-derived stem cellsosteogenic differentiation
Publisher
IOP PUBLISHING LTD
Indexed
SCIE; SCOPUS WOS scopus
Abstract
The aim of this study is to fabricate lactoferrin (LF)-carrying hydroxyapatite nanoparticles (HAp NPs) to enhance osteogenic differentiation of rabbit adipose-derived stem cells (rADSCs). HAp NPs were modified with heparin-dopamine (Hep-DOPA) (Hep-HAp) and further immobilized with LF (LF/Hep-HAp). Heparin immobilization on HAp NPs prevented aggregation of HAp NPs in aqueous solution and prolonged the release of LF from LF/Hep-HAp NPs. In vitro studies of rADSCs have demonstrated that LF-Hep/HAp NPs significantly increase alkaline phosphatase (ALP) activity, calcium deposition, and both mRNA expression of osteocalcin (OCN) and osteopontin (OPN) in comparison with HAp and Hep-HAp NPs. These results suggest that LF/Hep-HAp NPs can effectively induce osteogenic differentiation of rADSCs.
DOI
10.1088/1748-6041/11/2/025004
Appears in Collections:
엘텍공과대학 > 식품공학전공 > Journal papers
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