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Myogenic differentiation potential of human tonsil-derived mesenchymal stem cells and their potential for use to promote skeletal muscle regeneration

Title
Myogenic differentiation potential of human tonsil-derived mesenchymal stem cells and their potential for use to promote skeletal muscle regeneration
Authors
Park, SaeyoungChoi, YoonyoungJung, NamheeYu, YeonsilRyu, Kyung-HaKim, Han SuJo, InhoChoi, Byung-OkJung, Sung-Chul
Ewha Authors
유경하김한수정성철조인호유연실박세영
SCOPUS Author ID
유경하scopus; 김한수scopus; 정성철scopus; 조인호scopus; 유연실scopus; 박세영scopus
Issue Date
2016
Journal Title
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
ISSN
1107-3756JCR Link1791-244XJCR Link
Citation
vol. 37, no. 5, pp. 1209 - 1220
Keywords
human tonsil-derived mesenchymal stem cellsskeletal muscledifferentiationregenerationstem cell therapy
Publisher
SPANDIDOS PUBL LTD
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
Stem cells are regarded as an important source of cells which may be used to promote the regeneration of skeletal muscle (SKM) which has been damaged due to defects in the organization of muscle tissue caused by congenital diseases, trauma or tumor removal. In particular, mesenchymal stem cells (MSCs), which require less invasive harvesting techniques, represent a valuable source of cells for stem cell therapy. In the present study, we demonstrated that human tonsil-derived MSCs (T-MSCs) may differentiate into myogenic cells in vitro and that the transplantation of myoblasts and myocytes generated from human T-MSCs mediates the recovery of muscle function in vivo. In order to induce myogenic differentiation, the T-MSC-derived spheres were cultured in Dulbecco's modified Eagle's medium/nutrient mixture F-12 (DMEM/F-12) supplemented with 1 ng/ml transforming growth factor-, non-essential amino acids and insulin-transferrin-selenium for 4 days followed by culture in myogenic induction medium [low-glucose DMEM containing 2% fetal bovine serum (FBS) and 10 ng/ml insulin-like growth factor 1 (IGF1)] for 14 days. The T-MSCs sequentially differentiated into myoblasts and skeletal myocytes, as evidenced by the increased expression of skeletal myogenesis-related markers [including -actinin, troponin I type 1 (TNNI1) and myogenin] and the formation of myotubes in vitro. The in situ transplantation of T-MSCs into mice with a partial myectomy of the right gastrocnemius muscle enhanced muscle function, as demonstrated by gait assessment (footprint analysis), and restored the shape of SKM without forming teratomas. Thus, T-MSCs may differentiate into myogenic cells and effectively regenerate SKM following injury. These results demonstrate the therapeutic potential of T-MSCs to promote SKM regeneration following injury.
DOI
10.3892/ijmm.2016.2536
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의과대학 > 의학과 > Journal papers
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