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Bioreducible Cationic Poly(amido amine)s for Enhanced Gene Delivery and Osteogenic Differentiation of Tonsil-Derived Mesenchymal Stem Cells

Title
Bioreducible Cationic Poly(amido amine)s for Enhanced Gene Delivery and Osteogenic Differentiation of Tonsil-Derived Mesenchymal Stem Cells
Authors
Jeong, HansaemLee, Eun-SeoJung, GiyoungPark, JunghaJeong, ByeongmoonRyu, Kyung HaHwang, Nathaniel S.Lee, Hyukjin
Ewha Authors
유경하정병문이혁진
SCOPUS Author ID
유경하scopus; 정병문scopus; 이혁진scopus
Issue Date
2016
Journal Title
JOURNAL OF BIOMEDICAL NANOTECHNOLOGY
ISSN
1550-7033JCR Link1550-7041JCR Link
Citation
vol. 12, no. 5, pp. 1023 - 1034
Keywords
Non-Viral VectorGene Delivery SystemOsteogenesisTonsil-Derived Mesenchymal Stem Cells
Publisher
AMER SCIENTIFIC PUBLISHERS
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
The development of efficient and safe gene delivery carriers has been a major challenge in the clinical application of non viral gene therapy. Herein, we report novel bioreducible poly(amido amine)s for the efficient delivery of genetic material such as plasmid DNA. A library of 34 different bioreducible polymer compounds was synthesized and screened to find lead materials for in vitro gene transfection. Our lead material (CBA-106) allows effortless polyplex formation with genetic materials by electrostatic interactions at the weight ratio of 1:5 (DNA/polymer). Polyplexes were further characterized by DLS and AFM analysis. Enhanced serum stability and bioreducibility under physiological conditions were confirmed, in addition to low cellular cytotoxicity. When compared with a commercially available gene delivery carrier (Lipofectamine(R) 2000), CBA-106 shows comparable or even surpassing gene transfection efficiency. Furthermore, BMP-2 plasmids were efficiently delivered to tonsil-derived mesenchymal stem cells (TMSCs) for osteogenic commitment in vitro and in vivo. Taken together, our results clearly demonstrate the potential of novel bioreducible polymeric systems for gene delivery applications. We suggest that our system can provide a valuable platform for the broad application of gene regulation in cell therapy and regenerative medicine.
DOI
10.1166/jbn.2016.2223
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의학전문대학원 > 의학과 > Journal papers
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