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Activation of classical estrogen receptor subtypes reduces tight junction disruption of brain endothelial cells under ischemia/reperfusion injury

Title
Activation of classical estrogen receptor subtypes reduces tight junction disruption of brain endothelial cells under ischemia/reperfusion injury
Authors
Shin, Jin A.Yoon, Joo ChunKim, MinsukPark, Eun-Mi
Ewha Authors
박은미윤주천김민석신진아
SCOPUS Author ID
박은미scopus; 윤주천scopus; 김민석scopus; 신진아scopus
Issue Date
2016
Journal Title
FREE RADICAL BIOLOGY AND MEDICINE
ISSN
0891-5849JCR Link1873-4596JCR Link
Citation
vol. 92, pp. 78 - 89
Keywords
Blood-brain barrierEndothelial cellEstrogen receptorIschemic reperfusion injuryParacellular permeabilityTight junction
Publisher
ELSEVIER SCIENCE INC
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
Ischemic stroke, which induces oxidative stress in the brain, disrupts tight junctions (TJs) between brain endothelial cells, resulting in blood-brain barrier (BBB) breakdown and brain edema. Estrogen reduces oxidative stress and protects brain endothelial cells from ischemic insult. The aim of this study was to determine the protective effects of estrogen on TJ disruption and to examine the roles of classical estrogen receptor (ER) subtypes, ER alpha- and ER beta, in estrogen effects in brain endothelial cells (bEnd.3) exposed to oxygen-glucose deprivation/reperfusion (OGD/R) injury. Estrogen pretreatment prevented OGD/R-induced decreases in cell viability and TJ protein levels. ER alpha- and ER beta-specific agonists also reduced TJ disruption. Knockdown of ER alpha or ER beta expression partially inhibited the effects of estrogen, but completely reversed the effects of corresponding ER subtype-specific agonists on the outcomes of OGD/R. During the early reperfusion period, activation of extracellular signal-regulated kinase1/2 and hypoxia-inducible factor 1 alpha/vascular endothelial growth factor was associated with decreased expression of occludin and claudin-5, respectively, and these changes in TJ protein levels were differentially regulated by ER subtype-specific agonists. Our results suggest that ERA and EV activation reduce TJ disruption via inhibition of signaling molecules after ischemic injury and that targeting each ER subtype can be a useful strategy for protecting the BBB from ischemic stroke in postmenopausal women. (C) 2016 Elsevier Inc. All rights reserved.
DOI
10.1016/j.freeradbiomed.2016.01.010
Appears in Collections:
의과대학 > 의학과 > Journal papers
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