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Stable G protein-effector complexes in striatal neurons: mechanism of assembly and role in neurotransmitter signaling
- Title
- Stable G protein-effector complexes in striatal neurons: mechanism of assembly and role in neurotransmitter signaling
- Authors
- Xie, Keqiang; Masuho, Ikuo; Shih, Chien-Cheng; Cao, Yan; Sasaki, Keita; Lai, Chun Wan J.; Han, Pyung-Lim; Ueda, Hiroshi; Dessauer, Carmen W.; Ehrlich, Michelle E.; Xu, Baoji; Willardson, Barry M.; Martemyanov, Kirill A.
- Ewha Authors
- 한평림
- SCOPUS Author ID
- 한평림
- Issue Date
- 2015
- Journal Title
- ELIFE
- ISSN
- 2050-084X
- Citation
- ELIFE vol. 4
- Publisher
- ELIFE SCIENCES PUBLICATIONS LTD
- Indexed
- SCIE; SCOPUS
- Document Type
- Article
- Abstract
- In the striatum, signaling via G protein-coupled neurotransmitter receptors is essential for motor control. Critical to this process is the effector enzyme adenylyl cyclase type 5 (AC5) that produces second messenger cAMP upon receptor-mediated activation by G protein Golf. However, the molecular organization of the G(olf)-AC5 signaling axis is not well understood. In this study, we report that in the striatum AC5 exists in a stable pre-coupled complex with subunits of G(olf) heterotrimer. We use genetic mouse models with disruption in individual components of the complex to reveal hierarchical order of interactions required for AC5-G(olf) stability. We further identify that the assembly of AC5-G(olf) complex is mediated by PhLP1 chaperone that plays central role in neurotransmitter receptor coupling to cAMP production motor learning. These findings provide evidence for the existence of stable G protein-effector signaling complexes and identify a new component essential for their assembly.
- DOI
- 10.7554/eLife.10451
- Appears in Collections:
- 일반대학원 > 뇌·인지과학과 > Journal papers
- Files in This Item:
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