View : 25 Download: 12

Stable G protein-effector complexes in striatal neurons: mechanism of assembly and role in neurotransmitter signaling

Title
Stable G protein-effector complexes in striatal neurons: mechanism of assembly and role in neurotransmitter signaling
Authors
Xie, KeqiangMasuho, IkuoShih, Chien-ChengCao, YanSasaki, KeitaLai, Chun Wan J.Han, Pyung-LimUeda, HiroshiDessauer, Carmen W.Ehrlich, Michelle E.Xu, BaojiWillardson, Barry M.Martemyanov, Kirill A.
Ewha Authors
한평림
SCOPUS Author ID
한평림scopus
Issue Date
2015
Journal Title
ELIFE
ISSN
2050-084XJCR Link
Citation
vol. 4
Publisher
ELIFE SCIENCES PUBLICATIONS LTD
Indexed
SCIE; SCOPUS WOS scopus
Abstract
In the striatum, signaling via G protein-coupled neurotransmitter receptors is essential for motor control. Critical to this process is the effector enzyme adenylyl cyclase type 5 (AC5) that produces second messenger cAMP upon receptor-mediated activation by G protein Golf. However, the molecular organization of the G(olf)-AC5 signaling axis is not well understood. In this study, we report that in the striatum AC5 exists in a stable pre-coupled complex with subunits of G(olf) heterotrimer. We use genetic mouse models with disruption in individual components of the complex to reveal hierarchical order of interactions required for AC5-G(olf) stability. We further identify that the assembly of AC5-G(olf) complex is mediated by PhLP1 chaperone that plays central role in neurotransmitter receptor coupling to cAMP production motor learning. These findings provide evidence for the existence of stable G protein-effector signaling complexes and identify a new component essential for their assembly.
DOI
10.7554/eLife.10451
Appears in Collections:
일반대학원 > 뇌·인지과학과 > Journal papers
Files in This Item:
Stable G protein-effector complexes in striatal neurons mechanism of assembly and role in neurotransmitter signaling.pdf(3.19 MB)Download
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE