View : 27 Download: 0

Ultrasonication as a rapid and high yield DNA extraction method for bacterial gene quantification by NanoGene assay

Title
Ultrasonication as a rapid and high yield DNA extraction method for bacterial gene quantification by NanoGene assay
Authors
Wang, XiaofangCho, Kyung-SukSon, Ahjeong
Ewha Authors
조경숙손아정
SCOPUS Author ID
조경숙scopus; 손아정scopus
Issue Date
2015
Journal Title
BIOTECHNOLOGY AND BIOPROCESS ENGINEERING
ISSN
1226-8372JCR Link1976-3816JCR Link
Citation
vol. 20, no. 6, pp. 1133 - 1140
Keywords
ultrasonicationNanoGene assaybacterial gene quantificationhumic acidssand samplesDNA extraction
Publisher
KOREAN SOC BIOTECHNOLOGY & BIOENGINEERING
Indexed
SCIE; SCOPUS; KCI WOS scopus
Abstract
We demonstrated ultrasonication as a rapid and high yield DNA extraction method suitable for bacterial gene quantification by NanoGene assay. The NanoGene assay utilizes DNA hybridization in solution and a combination of magnetic beads and quantum dot nanoparticles. Unlike the existing gene quantification assays, the NanoGene method is capable of quantifying genes in the presence of environmental inhibitors and cell materials. The performance of the ultrasonication was compared with heating and freeze-thaw. They first were evaluated for their cell lysis capability in humic acids laden sand samples, via EtBr assay. Using autoclaved samples as a bench mark, their cell lysis capability were 106 +/- 3, 68 +/- 5, and 48 +/- 15%, respectively. Morphological changes of cells for each method were also observed by FE-SEM. More importantly, ultrasonication performed significantly better (more than 3x fluorescence signal) than commercial DNA extraction methods during bacterial gene quantification in humic acids laden sand samples.
DOI
10.1007/s12257-015-0465-6
Appears in Collections:
엘텍공과대학 > 환경공학전공 > Journal papers
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE