View : 124 Download: 0

DEVELOPMENT OF A DNA MICROARRAY-BASED IDENTIFICATION SYSTEM FOR COMMERCIALLY IMPORTANT KOREAN OYSTER SPECIES

Title
DEVELOPMENT OF A DNA MICROARRAY-BASED IDENTIFICATION SYSTEM FOR COMMERCIALLY IMPORTANT KOREAN OYSTER SPECIES
Authors
Kim, JiyeonJung, DaewuiLee, Won SunPark, Joong-Ki
Ewha Authors
박중기
SCOPUS Author ID
박중기scopus
Issue Date
2015
Journal Title
JOURNAL OF SHELLFISH RESEARCH
ISSN
0730-8000JCR Link1943-6319JCR Link
Citation
vol. 34, no. 3, pp. 841 - 847
Keywords
DNA microarraymolecular identificationoysterCrassostreaOstreamitochondrial cox1
Publisher
NATL SHELLFISHERIES ASSOC
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
The correct identification of oyster species is essential for both aquaculture and taxonomic study, but it has often been a challenging task due to enormous morphological variation in shell morphs with ecophenotypic origins. The difficulty of species identification based entirely on shell characters has raised the need for developing an accurate, rapid tool for the identification and discrimination of oyster species. In this study, a DNA microarray-based identification system was established for eight commercially important oyster species (Crassostrea ariakensis, Crassostrea gigas, Crassostrea sikamea, Crassostrea nippona, Crassostrea angulata, Ostrea circumpicta, Ostrea denselamellosa, and Saccostrea kegaki). Sixteen species-specific probes developed in this study unambiguously distinguished eight target oyster species with no false-positive or false-negative signals. Of the eight oyster species examined, three Crassostrea species, C. angulata, C. gigas, and C. sikamea, which are indistinguishable by morphology, could be precisely identified using species-specific hybridization probes. The DNA microarray-based identification system developed in this study offers a very effective and reliable tool for eight oyster species, most of which are of commercial value and/or ecological significance in the macrobenthic community of offshore marine environments.
DOI
10.2983/035.034.0313
Appears in Collections:
일반대학원 > 에코과학부 > Journal papers
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE