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Macaca specific exon creation event generates a novel ZKSCAN5 transcript

Title
Macaca specific exon creation event generates a novel ZKSCAN5 transcript
Authors
Kim, Young-HyunChoe, Se-HeeSong, Bong-SeokPark, Sang-JeKim, Myung-JinPark, Young-HoYoon, Seung-BinLee, YoungjeonJin, Yeung BaeSim, Bo-WoongKim, Ji-SuJeong, Kang-JinKim, Sun-UkLee, Sang-RaePark, Young-IlHuh, Jae-WonChang, Kyu-Tae
Ewha Authors
박영일
SCOPUS Author ID
박영일scopus
Issue Date
2016
Journal Title
GENE
ISSN
0378-1119JCR Link1879-0038JCR Link
Citation
vol. 577, no. 2, pp. 236 - 243
Keywords
Alternative splicingMacacaExon creationLineage specificZKSCAN5
Publisher
ELSEVIER SCIENCE BV
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
ZKSCAN5 (also known as ZFP95) is a zinc-finger protein belonging to the Kruppel family. ZKSCAN5 contains a SCAN box and a KRAB A domain and is proposed to play a distinct role during spermatogenesis. In humans, alternatively spliced ZKSCAN5 transcripts with different 5'-untranslated regions (UTRs) have been identified. However, investigation of our Macaca UniGene Database revealed novel alternative ZKSCAN5 transcripts that arose due to an exon creation event. Therefore, in this study, we identified the full-length sequences of ZKSCAN5 and its alternative transcripts in Macaca spp. Additionally, we investigated different nonhuman primate sequences to elucidate the molecular mechanism underlying the exon creation event. We analyzed the evolutionary features of the ZKSCAN5 transcripts by reverse transcription polymerase chain reaction (RT-PCR) and genomic PCR, and by sequencing various nonhuman primate DNA and RNA samples. The exon-created transcript was only detected in the Macaca lineage (crab-eating monkey and rhesus monkey). Full-length sequence analysis by rapid amplification of cDNA ends (RACE) identified ten full-length transcripts and four functional isoforms of ZKSCAN5. Protein sequence analyses revealed the presence of two groups of isoforms that arose because of differences in start-codon usage. Together, our results demonstrate that there has been specific selection for a discrete set of ZKSCAN5 variants in the Macaca lineage. Furthermore, study of this locus (and perhaps others) in Macaca spp. might facilitate our understanding of the evolutionary pressures that have shaped the mechanism of exon creation in primates. (C) 2015 The Authors. Published by Elsevier B.V.
DOI
10.1016/j.gene.2015.11.051
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신산업융합대학 > 융합콘텐츠학과 > Journal papers
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