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Generation of a human antibody that inhibits TSPAN8-mediated invasion of metastatic colorectal cancer cells

Title
Generation of a human antibody that inhibits TSPAN8-mediated invasion of metastatic colorectal cancer cells
Authors
Kim, Taek-KeunPark, Chang SikJeoung, Mee HyunLee, Woo RanGo, Nam KyungChoi, Jong RipLee, Tae SupShim, HyunboLee, Sukmook
Ewha Authors
심현보
SCOPUS Author ID
심현보scopus
Issue Date
2015
Journal Title
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
ISSN
0006-291XJCR Link1090-2104JCR Link
Citation
vol. 468, no. 4, pp. 774 - 780
Keywords
TSPAN8Large extracellular loopHuman antibodyColorectal cancer cellsInvasion
Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
Tetraspanin 8 (TSPAN8) is a tumor-associated antigen implicated in tumor progression and metastasis. However, the validation of TSPAN8 as a potential therapeutic target in metastatic colorectal cancer (mCRC) has not yet been studied. In this study, through several in vitro methodologies, we identified a large extracellular loop of TSPAN8 (TSPAN8-LEL) as a key domain for regulating mCRC invasion. Using phage display technology, we developed a novel anti-TSPAN8-LEL human antibody with subnanomolar affinity that specifically recognizes amino acids 140-205 of TSPAN8-LEL in a conformation-dependent manner. Finally, we demonstrated that the antibody specifically reduces invasion in the HCT116 and LoVo mCRC cell lines more potently than in the HCT-8 and SW480 non-mCRC cell lines. Our data suggest that TSPAN8-LEL may play an important role in mCRC cell invasion, and that the antibody we have developed could be a useful tool for inhibiting the invasion of TSPAN8-expressing mCRCs. (C) 2015 Elsevier Inc. All rights reserved.
DOI
10.1016/j.bbrc.2015.11.031
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일반대학원 > 바이오융합과학과 > Journal papers
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