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Chemical approach to solvent removal during nanoencapsulation: its application to preparation of PLGA nanoparticles with non-halogenated solvent

Title
Chemical approach to solvent removal during nanoencapsulation: its application to preparation of PLGA nanoparticles with non-halogenated solvent
Authors
Lee, YoungmeSah, EricSah, Hongkee
Ewha Authors
사홍기
SCOPUS Author ID
사홍기scopus
Issue Date
2015
Journal Title
JOURNAL OF NANOPARTICLE RESEARCH
ISSN
1388-0764JCR Link1572-896XJCR Link
Citation
vol. 17, no. 11
Keywords
Poly(lactide-co-glycolide)NanoparticlesNanoencapsulationSolvent removalDrug deliveryNanomedicine
Publisher
SPRINGER
Indexed
SCIE; SCOPUS WOS scopus
Abstract
The objective of this study was to develop a new oil-in-water emulsion-based nanoencapsulation method for the preparation of PLGA nanoparticles using a non-halogenated solvent. PLGA (60-150 mg) was dissolved in 3 ml of methyl propionate, which was vortexed with 4 ml of a 0.5-4 % polyvinyl alcohol solution. This premix was sonicated for 2 min, added into 30 ml of the aqueous polyvinyl alcohol solution, and reacted with 3 ml of 10 N NaOH. Solvent removal was achieved by the alkaline hydrolysis of methyl propionate dissolved in an aqueous phase into water-soluble methanol and sodium propionate. It was a simple but effective technique to quickly harden nanoemulsion droplets into nanoparticles. The appearing PLGA nanoparticles were recovered by ultracentrifugation and/or dialysis, lyophilized with trehalose, and redispersed by water. This nanoencapsulation technique permitted a control of their mean diameters over 151.7 +/- 3.8 to 440.2 +/- 22.2 nm at mild processing conditions. When the aqueous polyvinyl alcohol concentration was set at >= 1 %, nanoparticles showed uniform distributions with polydispersity indices below 0.1. There were no significant changes in their mean diameters and size distribution patterns before and after lyophilization. When mestranol was encapsulated into nanoparticles, the drug was completely nanoencapsulated: depending on experimental conditions, their encapsulation efficiencies were determined to be 99.4 +/- 7.2 to 105.8 +/- 6.3 %. This simple, facile nanoencapsulation technique might have versatile applications for the preparation of polymeric nanoparticulate dosage forms.
DOI
10.1007/s11051-015-3262-4
Appears in Collections:
약학대학 > 약학과 > Journal papers
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