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Hydrogen-deuterium exchange mass spectrometry for determining protein structural changes in drug discovery

Title
Hydrogen-deuterium exchange mass spectrometry for determining protein structural changes in drug discovery
Authors
Lee, Jae-JinPark, Yeon SeungLee, Kong-Joo
Ewha Authors
이공주이제진
SCOPUS Author ID
이공주scopus; 이제진scopus
Issue Date
2015
Journal Title
ARCHIVES OF PHARMACAL RESEARCH
ISSN
0253-6269JCR Link1976-3786JCR Link
Citation
vol. 38, no. 10, pp. 1737 - 1745
Keywords
Hydrogen-deuterium exchange (HDX)Mass spectrometry (MS)Protein structure changeProtein-protein and -chemical interactionNm23-H1UCH-L1
Publisher
PHARMACEUTICAL SOC KOREA
Indexed
SCIE; SCOPUS; KCI WOS scopus
Abstract
Protein structures are dynamically changed in response to post-translational modifications, ligand or chemical binding, or protein-protein interactions. Understanding the structural changes that occur in proteins in response to potential candidate drugs is important for predicting the modes of action of drugs and their functions and regulations. Recent advances in hydrogen/deuterium exchange mass spectrometry (HDX-MS) have the potential to offer a tool for obtaining such understanding similarly to other biophysical techniques, such as X-ray crystallography and high resolution NMR. We present here, a review of basic concept and methodology of HDX-MS, how it is being applied for identifying the sites and structural changes in proteins following their interactions with other proteins and small molecules, and the potential of this tool to help in drug discovery.
DOI
10.1007/s12272-015-0584-9
Appears in Collections:
약학대학 > 약학과 > Journal papers
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