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Estimation of multiexponential fluorescence decay parameters using compressive sensing

Title
Estimation of multiexponential fluorescence decay parameters using compressive sensing
Authors
Yang, SejungLee, JoohyunLee, YouminLee, MinyungLee, Byung-Uk
Ewha Authors
이민영이병욱양세정
SCOPUS Author ID
이민영scopus; 이병욱scopus; 양세정scopus
Issue Date
2015
Journal Title
JOURNAL OF BIOMEDICAL OPTICS
ISSN
1083-3668JCR Link1560-2281JCR Link
Citation
vol. 20, no. 9
Keywords
fluorescence lifetime imaging microscopymultiexponential decayfluorescence decay parameterscompressive sensing
Publisher
SPIE-SOC PHOTO-OPTICAL INSTRUMENTATION ENGINEERS
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
Fluorescence lifetime imaging microscopy (FLIM) is a microscopic imaging technique to present an image of fluorophore lifetimes. It circumvents the problems of typical imaging methods such as intensity attenuation from depth since a lifetime is independent of the excitation intensity or fluorophore concentration. The lifetime is estimated from the time sequence of photon counts observed with signal-dependent noise, which has a Poisson distribution. Conventional methods usually estimate single or biexponential decay parameters. However, a lifetime component has a distribution or width, because the lifetime depends on macromolecular conformation or inhomogeneity. We present a novel algorithm based on a sparse representation which can estimate the distribution of lifetime. We verify the enhanced performance through simulations and experiments. (C) The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.
DOI
10.1117/1.JBO.20.9.096003
Appears in Collections:
자연과학대학 > 화학·나노과학전공 > Journal papers
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