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Characterisation of insulin-producing cells differentiated from tonsil derived mesenchymal stem cells

Title
Characterisation of insulin-producing cells differentiated from tonsil derived mesenchymal stem cells
Authors
Kim, So-YeonKim, Ye-RyungPark, Woo-JaeKim, Han SuJung, Sung-ChulWoo, So-YounJo, InhoRyu, Kyung-HaPark, Joo-Won
Ewha Authors
유경하우소연김한수정성철조인호박주원
SCOPUS Author ID
유경하scopus; 우소연scopus; 김한수scopus; 정성철scopus; 조인호scopus; 박주원scopus
Issue Date
2015
Journal Title
DIFFERENTIATION
ISSN
0301-4681JCR Link1432-0436JCR Link
Citation
vol. 90, no. 42007, pp. 27 - 39
Keywords
InsulinDiabetesMesenchymal stem cellTonsilAdipose tissue
Publisher
ELSEVIER SCI LTD
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
Tonsil-derived (T-) mesenchymal stem cells (MSCs) display mutilineage differentiation potential and self-renewal capacity and have potential as a banking source. Diabetes mellitus is a prevalent disease in modern society, and the transplantation of pancreatic progenitor cells or various stem cell-derived insulin-secreting cells has been suggested as a novel therapy for diabetes. The potential of T-MSCs to trans-differentiate into pancreatic progenitor cells or insulin-secreting cells has not yet been investigated. We examined the potential of human T-MSCs to trans-differentiate into pancreatic islet cells using two different methods based on beta-mercaptoethanol and insulin-transferin-selenium, respectively. First, we compared the efficacy of the two methods for inducing differentiation into insulin-producing cells. We demonstrated that the insulin-transferin-selenium method is more efficient for inducing differentiation into insulin-secreting cells regardless of the source of the MSCs. Second, we compared the differentiation potential of two different MSC types: T-MSCs and adipose-derived MSCs (A-MSCs). T-MSCs had a differentiation capacity similar to that of A-MSCs and were capable of secreting insulin in response to glucose concentration. Islet-like clusters differentiated from T-MSCs had lower synaptotagmin-3, -5, -7, and -8 levels, and consequently lower secreted insulin levels than cells differentiated from A-MSCs. These results imply that T-MSCs can differentiate into functional pancreatic islet-like cells and could provide a novel, alternative cell therapy for diabetes mellitus. (C) 2015 International Society of Differentiation. Published by Elsevier B.V. All rights reserved.
DOI
10.1016/j.diff.2015.08.001
Appears in Collections:
의학전문대학원 > 의학과 > Journal papers
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