Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 조인호 | * |
dc.contributor.author | 안정혁 | * |
dc.contributor.author | 이현주 | * |
dc.contributor.author | 박정현 | * |
dc.date.accessioned | 2016-08-27T04:08:08Z | - |
dc.date.available | 2016-08-27T04:08:08Z | - |
dc.date.issued | 2015 | * |
dc.identifier.issn | 1089-8603 | * |
dc.identifier.issn | 1089-8611 | * |
dc.identifier.other | OAK-15627 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/217601 | - |
dc.description.abstract | DNA damage is significant in endothelial cells (EC), particularly in anticancer chemotherapy. Here, we explored whether and how aphidicolin, a DNA-damaging chemical with a promising anticancer activity, alters NO production in bovine aortic endothelial cells (BAEC). In addition to increasing eNOS-Ser(1179) phosphorylation, aphidicolin decreased eNOS-Ser(116) phosphorylation with a concomitant increase in NO production in a time-dependent manner. The amino acid sequence around the eNOS-Ser116 residue was identified as the substrate site of the regulatory subunit B56 delta of protein phosphatase 2A (PP2A). As expected, okadaic acid, a specific PP2A inhibitor, reversed aphidicolin-induced eNOS-Ser(116) dephosphorylation in a dose-dependent manner. Aphidicolin also increased B56 delta-Ser(566) phosphorylation, although expression of neither the catalytic subunit C alpha (PP2A C alpha) nor B56 delta was altered. Ectopic expression of dominant negative (dn)-B56 delta reversed all of the observed effects of aphidicolin with respect to phosphorylation of eNOS-Ser(116) and B56 delta-Ser(566). Lastly, aphidicolin-stimulated NO production was also partially attenuated by ectopic expression of dn-B56 delta. Taken together, our results are the first to demonstrate that aphidicolin decreases phosphorylation of eNOS-Ser(116), at least in part by activating PP2A B56 delta, resulting in NO release in BAEC. (C) 2015 Elsevier Inc. All rights reserved. | * |
dc.language | English | * |
dc.publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE | * |
dc.subject | Endothelial nitric oxide synthase | * |
dc.subject | Aphidicolin | * |
dc.subject | Phosphorylation | * |
dc.subject | Protein phosphatase 2A | * |
dc.subject | PP2A B56 delta subunit | * |
dc.title | B56 delta subunit of protein phosphatase 2A decreases phosphorylation of endothelial nitric oxide synthase at serine 116: Mechanism underlying aphidicolin-stimulated NO production | * |
dc.type | Article | * |
dc.relation.volume | 50 | * |
dc.relation.index | SCI | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 46 | * |
dc.relation.lastpage | 51 | * |
dc.relation.journaltitle | NITRIC OXIDE-BIOLOGY AND CHEMISTRY | * |
dc.identifier.doi | 10.1016/j.niox.2015.08.001 | * |
dc.identifier.wosid | WOS:000363362900006 | * |
dc.identifier.scopusid | 2-s2.0-84941202537 | * |
dc.author.google | Park, Jung-Hyun | * |
dc.author.google | Cho, Du-Hyong | * |
dc.author.google | Lee, Jee Young | * |
dc.author.google | Lee, Hyeon-Ju | * |
dc.author.google | Ha, Yena | * |
dc.author.google | Ahn, Jung-Hyuck | * |
dc.author.google | Jo, Inho | * |
dc.contributor.scopusid | 조인호(26643129000;56663841900) | * |
dc.contributor.scopusid | 안정혁(35081632000) | * |
dc.contributor.scopusid | 이현주(57204537944) | * |
dc.contributor.scopusid | 박정현(56937220800;57157650200;57157679600) | * |
dc.date.modifydate | 20240123112949 | * |