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Quantification of Salmonella Typhimurium in liquid food using NanoGene assay

Title
Quantification of Salmonella Typhimurium in liquid food using NanoGene assay
Authors
Ahn, Joo-MyungSon, Ahjeong
Ewha Authors
손아정
SCOPUS Author ID
손아정scopus
Issue Date
2015
Journal Title
ANALYTICAL METHODS
ISSN
1759-9660JCR Link1759-9679JCR Link
Citation
vol. 7, no. 18, pp. 7674 - 7679
Publisher
ROYAL SOC CHEMISTRY
Indexed
SCIE; SCOPUS WOS scopus
Abstract
A food-borne pathogen, Salmonella Typhimurium, was detected and quantified in liquid food samples using NanoGene assay. The NanoGene assay is a recently developed gene quantification assay that employs DNA hybridization in solution and quantum dot nanoparticles. The invA gene was used as a functional gene target for the quantification of S. Typhimurium in liquid food samples (chicken broth, vegetable broth, and milk). We demonstrated quantification linearity with R-2 = 0.91. However, a reduced sensitivity suggested that the NanoGene assay might have limited inhibitions to certain food compounds. Food compounds namely glucose, sucrose, L-lysine, casein, minerals, and oil were examined to further identify their respective inhibitory contribution. It was shown that the NanoGene assay was highly resistant to inhibition by carbohydrates. However, it was vulnerable to proteins, in particular casein hydrolysate.
DOI
10.1039/c5ay01486g
Appears in Collections:
엘텍공과대학 > 환경공학전공 > Journal papers
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