View : 35 Download: 0

Boerhavia diffusa L. ethanol extract suppresses inflammatory responses via inhibition of Src/Syk/TRAF6

Title
Boerhavia diffusa L. ethanol extract suppresses inflammatory responses via inhibition of Src/Syk/TRAF6
Authors
Ha Van ThaiKim, EunjiKim, Seung CheolJeong, DeokYang, SungjaeBaek, Kwang-SooKim, YongRatan, Zubair AhmedYoon, Kee DongKim, Jong-HoonCho, Jae Youl
Ewha Authors
김승철
SCOPUS Author ID
김승철scopus
Issue Date
2015
Journal Title
JOURNAL OF FUNCTIONAL FOODS
ISSN
1756-4646JCR Link
Citation
vol. 17, pp. 476 - 490
Keywords
Boerhavia diffusa L. (Phyllanthaceae)Anti-inflammatory effectSrcSykTRAF6Luteolin
Publisher
ELSEVIER SCIENCE BV
Indexed
SCIE; SCOPUS WOS scopus
Abstract
Boerhavia diffusa L. is a green vegetable used as an herbal medicine in several Asian countries for the treatment of hepatitis, kidney stones, liver disorders, nephritis, urinary disorders, and urinary retention. In this study, the anti-inflammatory activity and the molecular inhibitory mechanisms of B. diffusa L. ethanol extract (Bd-EE) in vitro and in vivo were evaluated. Lipopolysaccharide (LPS)-activated peritoneal macrophage RAW264.7 cells and a mouse gastritis model induced by HCl/EtOH treatment were chosen to determine the in vitro and in vivo anti-inflammatory activities of Bd-EE. This extract (100 and 200 mu g/ml) significantly (P <0.01) inhibited nitric oxide (NO) and tumour necrosis factor (TNF)-alpha production in peritoneal macrophages and in RAW264.7 cells during LPS exposure at both. The inducible nitric oxide synthase (iNOS) and TNF-alpha mRNA expression levels were decreased. In addition, nuclear levels of p65, p50, c-Fos, and c-Jun transcription factors were reduced by Bd-EE treatment; moreover, it inhibited NF-kappa B upstream signalling via I kappa B alpha, PI3K, Syk, and Src. Furthermore, analysis of AP-1 upstream signalling revealed that the AP-1 activation pathway consisting of TRAF6, TAK1, MKK4/7, and MKK3/6 was also predominantly inhibited by Bd-EE. By HPLC analysis, luteolin was identified as a major ingredient displaying NO inhibitory activity. The data provided here strongly suggest that the anti-inflammatory property of Bd-EE is linked to the suppression of Syk, Src, and TRAF6. (C) 2015 Elsevier Ltd. All rights reserved.
DOI
10.1016/j.jff.2015.06.004
Appears in Collections:
의학전문대학원 > 의학과 > Journal papers
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE