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Induction of Angiogenesis by Matrigel Coating of VEGF-Loaded PEG/PCL-Based Hydrogel Scaffolds for hBMSC Transplantation

Title
Induction of Angiogenesis by Matrigel Coating of VEGF-Loaded PEG/PCL-Based Hydrogel Scaffolds for hBMSC Transplantation
Authors
Jung, Yeon JooKim, Kyung-ChulHeo, Jun-YoungJing, KaipengLee, Kyung EunHwang, Jun SeokLim, KyuJo, Deog-YeonAhn, Jae PyoungKim, Jin-ManHuh, Kang MooPark, Jong-Il
Ewha Authors
이경은
SCOPUS Author ID
이경은scopus
Issue Date
2015
Journal Title
MOLECULES AND CELLS
ISSN
1016-8478JCR Link0219-1032JCR Link
Citation
vol. 38, no. 7, pp. 663 - 668
Keywords
angiogenesiscontrolled releasehBMSChydrogelVEGF
Publisher
KOREAN SOC MOLECULAR & CELLULAR BIOLOGY
Indexed
SCI; SCIE; SCOPUS; KCI WOS
Abstract
hBMSCs are multipotent cells that are useful for tissue regeneration to treat degenerative diseases and others for their differentiation ability into chondrocytes, osteoblasts, adipocytes, hepatocytes and neuronal cells. In this study, biodegradable elastic hydrogels consisting of hydrophilic poly(ethylene glycol) (PEG) and hydrophobic poly(epsilon-caprolactone) (PCL) scaffolds were evaluated for tissue engineering because of its biocompatibility and the ability to control the release of bioactive peptides. The primary cultured cells from human bone marrow are confirmed as hBMSC by immunohistochemical analysis. Mesenchymal stem cell markers (collagen type I, fibronectin, CD54, integrin1 beta, and Hu protein) were shown to be positive, while hematopoietic stem cell markers (CD14 and CD45) were shown to be negative. Three different hydrogel scaffolds with different block compositions (PEG: PCL=6: 14 and 14: 6 by weight) were fabricated using the salt leaching method. The hBMSCs were expanded, seeded on the scaffolds, and cultured up to 8 days under static conditions in Iscove's Modified Dulbecco's Media (IMDM). The growth of MSCs cultured on the hydrogel with PEG/PCL=6/14 was faster than that of the others. In addition, the morphology of MSCs seemed to be normal and no cytotoxicity was found. The coating of the vascular endothelial growth factor (VEGF) containing scaffold with Matrigel slowed down the release of VEGF in vitro and promoted the angiogenesis when transplanted into BALB/c nude mice. These results suggest that hBMSCs can be supported by a biode gradable hydrogel scaffold for effective cell growth, and enhance the angiogenesis by Matrigel coating.
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의학전문대학원 > 의학과 > Journal papers
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