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Simultaneous quantitation and validation of method for the quality evaluation of Eucommiae cortex by HPLC/UV

Title
Simultaneous quantitation and validation of method for the quality evaluation of Eucommiae cortex by HPLC/UV
Authors
Zhao, Bing TianJeong, Su YangKim, Tae InSeo, Eun KyoungMin, Byung SunSon, Jong KeunWoo, Mi Hee
Ewha Authors
서은경
SCOPUS Author ID
서은경scopus
Issue Date
2015
Journal Title
ARCHIVES OF PHARMACAL RESEARCH
ISSN
0253-6269JCR Link1976-3786JCR Link
Citation
vol. 38, no. 12, pp. 2183 - 2192
Keywords
Eucommiae cortexHPLCPattern recognitionQuality control
Publisher
PHARMACEUTICAL SOC KOREA
Indexed
SCIE; SCOPUS; KCI WOS scopus
Abstract
A new HPLC/UV method has been developed for the simultaneous quantitative determination of four major components in Eucommiae cortex, namely geniposidic acid (1), geniposide (2), pinoresinol di-O-beta-d-glucopyranoside (3), and liriodendrin (4). Simultaneous separations of these four components were achieved on a J'sphere ODS C-18 column (250 x 4.6 mm, 4 A mu m). The elution was done using water with 0.1 % phosphoric acid (A) and acetonitrile with 0.1 % phosphoric acid (B) in a two-step elution of the mobile phase at a flow rate of 1.0 mL/min and a wavelength of 230 nm. The method was validated for linearity, recovery, precision, accuracy, stability and robustness. All calibration curves showed good linear regression (r (2) > 0.999) within the test ranges. This method showed good recovery and reproducibility for the quantification of these four components in 85 species of Eucommiae cortex. The intra-day and inter-day precisions were lower than 0.53 % (as a relative standard deviation, RSD) and accuracies between 93.00 and 106.28 % for all standards. The results indicate that the established HPLC/UV method is suitable for quantitation and quality evaluation of Eucommiae cortex.
DOI
10.1007/s12272-015-0642-3
Appears in Collections:
약학대학 > 약학과 > Journal papers
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