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Differentiated tonsil-derived mesenchymal stem cells embedded in Matrigel restore parathyroid cell functions in rats with parathyroidectomy

Title
Differentiated tonsil-derived mesenchymal stem cells embedded in Matrigel restore parathyroid cell functions in rats with parathyroidectomy
Authors
Park, Yoon ShinKim, Han SuJin, Yoon MiYu, YeonsilKim, Ha YeongPark, Hae SangJung, Sung-ChulHan, Ki-HwanPark, Yoon JeongRyu, Kyung-HaJo, Inho
Ewha Authors
유경하김한수정성철한기환조인호박윤신유연실
SCOPUS Author ID
유경하scopus; 김한수scopus; 정성철scopus; 한기환scopus; 조인호scopus; 박윤신scopusscopus; 유연실scopus
Issue Date
2015
Journal Title
BIOMATERIALS
ISSN
0142-9612JCR Link1878-5905JCR Link
Citation
vol. 65, pp. 140 - 152
Keywords
Parathyroid cellsParathyroid hormone (PTH)Tonsil-derived mesenchymal stem cells (TMSC)Parathyroidectomy (PTX)Matrigel (MA)
Publisher
ELSEVIER SCI LTD
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
Parathyroid cells release parathyroid hormone (PTH), which controls calcium homeostasis. Loss of parathyroid cells results in hypoparathyroidism and consequent low-turnover bone disease. Here, we investigated whether our recently-established human tonsil-derived mesenchymal stem cells (TMSC) restore in vivo parathyroid cell function in rats with parathyroidectomy (PTX). Compared with undifferentiated control TMSC, TMSC differentiated with activin A and soluble sonic hedgehog induced a significant release of PTH as early as day 7, with increased PTH release occurring in response to lower calcium levels and vice versa. Released PTH increased osteocalcin expression and alizarin red S staining in preosteoblastic cells, indicating its functional activity. PTX rats fed calcium-free diet only survived for 10 days. Subcutaneous injection with TMSC alone did not increase their survival rates, regardless of differentiation. However, survival rates increased for up to 28 days in response to TMSC embedded in Matrigel (TMSC-MA), showing 40% and 80% in control and differentiated TMSC-MA, respectively. When compared with continuous increases by control TMSC-MA, stable levels of secreted PTH and serum ionized calcium were found in PTX rats with differentiated TMSC-MA. This is the first report that differentiated TMSC resemble parathyroid cells and, if embedded in Matrigel, restore in vivo parathyroid function. (C) 2015 Elsevier Ltd. All rights reserved.
DOI
10.1016/j.biomaterials.2015.06.044
Appears in Collections:
의과대학 > 의학과 > Journal papers
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