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Evaluation of Eye Irritation Potential of Solid Substance with New 3D Reconstructed Human Cornea Model, MCTT HCE (TM)

Title
Evaluation of Eye Irritation Potential of Solid Substance with New 3D Reconstructed Human Cornea Model, MCTT HCE (TM)
Authors
Jang, Won-heeJung, Kyoung-miYang, Hye-riLee, MiriJung, Haeng-SunLee, Su-HyonPark, MiyoungLim, Kyung-Min
Ewha Authors
임경민
SCOPUS Author ID
임경민scopus
Issue Date
2015
Journal Title
BIOMOLECULES & THERAPEUTICS
ISSN
1976-9148JCR Link2005-4483JCR Link
Citation
vol. 23, no. 4, pp. 379 - 385
Keywords
Eye irritationReconstructed cornea modelMCTT HCE (TM) modelProtocol refinement
Publisher
KOREAN SOC APPLIED PHARMACOLOGY
Indexed
SCIE; SCOPUS WOS scopus
Abstract
The eye irritation potential of drug candidates or pharmaceutical ingredients should be evaluated if there is a possibility of ocular exposure. Traditionally, the ocular irritation has been evaluated by the rabbit Draize test. However, rabbit eyes are more sensitive to irritants than human eyes, therefore substantial level of false positives are unavoidable. To resolve this species difference, several three-dimensional human corneal epithelial (HCE) models have been developed as alternative eye irritation test methods. Recently, we introduced a new HCE model, MCTT HCE (TM) which is reconstructed with non-transformed human corneal cells from limbal tissues. Here, we examined if MCTT HCE (TM) can be employed to evaluate eye irritation potential of solid substances. Through optimization of washing method and exposure time, treatment time was established as 10 min and washing procedure was set up as 4 times of washing with 10 mL of PBS and shaking in 30 mL of PBS in a beaker. With the established eye irritation test protocol, 11 solid substances (5 non-irritants, 6 irritants) were evaluated which demonstrated an excellent predictive capacity (100% accuracy, 100% specificity and 100% sensitivity). We also compared the performance of our test method with rabbit Draize test results and in vitro cytotoxicity test with 2D human corneal epithelial cell lines.
DOI
10.4062/biomolther.2015.004
Appears in Collections:
약학대학 > 약학과 > Journal papers
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