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Effective inactivation of Candida albicans biofilms by using supercritical carbon dioxide

Title
Effective inactivation of Candida albicans biofilms by using supercritical carbon dioxide
Authors
Park, Hyong SeokYang, JungwooChoi, Hee JungKim, Kyoung Heon
Ewha Authors
최희정
SCOPUS Author ID
최희정scopus
Issue Date
2015
Journal Title
BIOPROCESS AND BIOSYSTEMS ENGINEERING
ISSN
1615-7591JCR Link1615-7605JCR Link
Citation
vol. 38, no. 9, pp. 1731 - 1737
Keywords
SterilizationSupercritical carbon dioxideCandida albicansBiofilm
Publisher
SPRINGER
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
Present sterilization methods for biofilms in medical devices have limitations. Therefore, an alternative sterilization method using supercritical carbon dioxide (SC-CO2) was tested on Candida albicans biofilms. The effect of varying pressure, temperature, and treatment time on the inactivation of C. albicans spores in suspensions and in biofilms was examined. The parameters such as treatment time, pressure, and temperature that led to the complete inactivation of C. albicans biofilms ranged 5-20 min, 100-200 bar, and 35-45 A degrees C, respectively. Notably, treatment of SC-CO2 at either 100 bar and 40 A degrees C or 200 bar and 30 A degrees C induced complete inactivation of spores within 5 min. Furthermore, it was found that wet biofilms (0.4 %, w/w) had higher sensitivity to SC-CO2 than dried biofilms. Finally, spore inactivation was confirmed by confocal laser scanning microscopy. In this study, the use of a low-temperature SC-CO2 sterilization method was proven to be effective in fungal biofilm inactivation, and the moisture content of biofilms was revealed to be the key factor for biofilm inactivation.
DOI
10.1007/s00449-015-1414-7
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의학전문대학원 > 의학과 > Journal papers
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