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CHOP deficiency prevents methylglyoxal-induced myocyte apoptosis and cardiac dysfunction

Title
CHOP deficiency prevents methylglyoxal-induced myocyte apoptosis and cardiac dysfunction
Authors
Nam, Dae-HwanHan, Jung-HwaLee, Tae-JinShishido, TetsuroLim, Jae HyangKim, Geun-YoungWoo, Chang-Hoon
Ewha Authors
임재향
SCOPUS Author ID
임재향scopus
Issue Date
2015
Journal Title
JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY
ISSN
0022-2828JCR Link1095-8584JCR Link
Citation
vol. 85, pp. 168 - 177
Keywords
MethylglyoxalCHOPER stressDiabetesCardiac dysfunction
Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
Epidemiological studies indicate that methylglyoxal (MGO) plasma levels are closely linked to diabetes and the exacerbation of diabetic cardiovascular complications. Recently, it was established that endoplasmic reticulum (ER) stress importantly contributes to the pathogenesis of diabetes and its cardiovascular complications. The objective of this study was to explore the mechanism by which diabetes instigates cardiomyocyte apoptosis and cardiac dysfunction via MGO-mediated myocyte apoptosis. Intriguingly, the MGO activated unfolded protein response pathway accompanying apoptotic events, such as cleavages of PARP-1 and caspase-3. In addition, Western blot analysis revealed that MGO-induced myocyte apoptosis was inhibited by depletion of CHOP with siRNA against Ddit3, the gene name for rat CHOP. To investigate the physiologic roles of CHOP in vivo, glucose tolerance and cardiac dysfunction were assessed in CHOP-deficient mice. No significant difference was observed between CHOP KO and littermate naive controls in terms of the MGO-induced impairment of glucose tolerance. In contrast, myocyte apoptosis, inflammation, and cardiac dysfunction were significantly diminished in CHOP KO compared with littermate na ve controls. These results showed that CHOP is the key signal for myocyte apoptosis and cardiac dysfunction induced by MGO. These findings suggest a therapeutic potential of CHOP inhibition in the management of diabetic cardiovascular complications including diabetic cardiomyopathy. (C) 2015 Elsevier Ltd. All rights reserved.
DOI
10.1016/j.yjmcc.2015.05.016
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의학전문대학원 > 의학과 > Journal papers
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