Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 안영호 | * |
dc.date.accessioned | 2016-08-27T04:08:39Z | - |
dc.date.available | 2016-08-27T04:08:39Z | - |
dc.date.issued | 2015 | * |
dc.identifier.issn | 0006-291X | * |
dc.identifier.issn | 1090-2104 | * |
dc.identifier.other | OAK-15087 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/217300 | - |
dc.description.abstract | 8-Chloro-cyclic AMP (8-Cl-cAMP) is a cyclic AMP analog that induces growth inhibition and apoptosis in a broad spectrum of cancer cells. Previously, we found that 8-Cl-cAMP-induced growth inhibition is mediated by AMP-activated protein kinase (AMPK) as well as p38 mitogen-activated protein kinase (p38 MAPK). To identify downstream mediators of the 8-Cl-cAMP signaling, we performed co-immunoprecipitation combined with mass spectrometry using the anti-AMPK or p38 MAPK antibodies. Through this approach, SHC1 was identified as one of the binding partners of p38 MAPK. SHC1 phosphorylation was suppressed by 8-Cl-CAMP in HeLa and MCF7 cancer cells, which was mediated by its metabolites, 8-Cl-adenosine and 8-Cl-ATP; however, 8-Cl-cAMP showed no effect on SHC1 phosphorylation in normal human fibroblasts. SHC1 siRNA induced AMPK and p38 MAPK phosphorylation and growth inhibition in cancer cells, and SHC1 overexpression re-sensitized human foreskin fibroblasts to the 8-Cl-CAMP treatment. SHC1 phosphorylation was unaffected by Compound C (an AMPK inhibitor) and SB203580 (a p38 MAPK inhibitor), which suggests that SHC1 is upstream of AMPK and p38 MAPK in the 8-Cl-CAMP-stimulated signaling cascade. On the basis of these findings, we conclude that SHC1 functions as a sensor during the 8-Cl-cAMP-induced growth inhibition in SHC1-overexpressing cancer cells. (C) 2015 Elsevier Inc. All rights reserved. | * |
dc.language | English | * |
dc.publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE | * |
dc.subject | 8-Chloro-cyclic AMP | * |
dc.subject | SHC1 | * |
dc.subject | AMP-activated protein kinase | * |
dc.subject | p38 MAP kinase | * |
dc.subject | Cancer therapy | * |
dc.title | SHC1 sensitizes cancer cells to the 8-Cl-cAMP treatment | * |
dc.type | Article | * |
dc.relation.issue | 4 | * |
dc.relation.volume | 463 | * |
dc.relation.index | SCI | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 673 | * |
dc.relation.lastpage | 678 | * |
dc.relation.journaltitle | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | * |
dc.identifier.doi | 10.1016/j.bbrc.2015.05.123 | * |
dc.identifier.wosid | WOS:000358455300033 | * |
dc.identifier.scopusid | 2-s2.0-84936890404 | * |
dc.author.google | Choi, Ki Young | * |
dc.author.google | Cho, Young Jun | * |
dc.author.google | Kim, Jeong Seon | * |
dc.author.google | Ahn, Young-Ho | * |
dc.author.google | Hong, Seung Hwan | * |
dc.contributor.scopusid | 안영호(7202402440) | * |
dc.date.modifydate | 20240222132209 | * |