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Egg-specific expression of protein with DNA methyltransferase activity in the biocarcinogenic liver fluke Clonorchis sinensis

Title
Egg-specific expression of protein with DNA methyltransferase activity in the biocarcinogenic liver fluke Clonorchis sinensis
Authors
Kim, Seon-HeeCho, Hye-JeongSohn, Woon-MokAhn, Chun-SeobKong, YoonYang, Hyun-JongBae, Young-An
Ewha Authors
양현종
SCOPUS Author ID
양현종scopus
Issue Date
2015
Journal Title
PARASITOLOGY
ISSN
0031-1820JCR Link1469-8161JCR Link
Citation
vol. 142, no. 9, pp. 1228 - 1238
Keywords
Clonorchis sinensisDNA methylationDNMT25-methylcytosineDNA-dependent methyltransferaseegg development
Publisher
CAMBRIDGE UNIV PRESS
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
Despite recent reports regarding the biology of cytosine methylation in Schistosoma mansoni, the impact of the regulatory machinery remains unclear in diverse platyhelminthes. This ambiguity is reinforced by discoveries of DNA methyltransferase 2 (DNMT2)-only organisms and the substrate specificity of DNMT2 preferential to RNA molecules. Here, we characterized a novel DNA methyltransferase, named CsDNMT2, in a liver fluke Clonorchis sinensis. The protein exhibited structural properties conserved in other members of the DNMT2 family. The native and recombinant CsDNMT2 exhibited considerable enzymatic activity on DNA. The spatiotemporal expression of CsDNMT2 mirrored that of 5-methylcytosine (5 mC), both of which were elevated in the C. sinensis eggs. However, CsDNMT2 and 5 mC were marginally detected in other histological regions of C. sinensis adults including ovaries and seminal receptacle. The methylation site seemed not related to genomic loci occupied by progenies of an active long-terminal-repeat retrotransposon. Taken together, our data strongly suggest that C. sinensis has preserved the functional DNA methylation machinery and that DNMT2 acts as a genuine alternative to DNMT1/DNMT3 to methylate DNA in the DNMT2-only organism. The epigenetic regulation would target functional genes primarily involved in the formation and/or maturation of eggs, rather than retrotransposons.
DOI
10.1017/S0031182015000566
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의학전문대학원 > 의학과 > Journal papers
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