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Proteomic analysis of fetal programming-related obesity markers

Title
Proteomic analysis of fetal programming-related obesity markers
Authors
Lee, Ji HyeYoo, Jae YoungYou, Young-AhKwon, Woo-SungLee, Sang MiPang, Myung-GeolKim, Young Ju
Ewha Authors
김영주유영아
SCOPUS Author ID
김영주scopus; 유영아scopus
Issue Date
2015
Journal Title
PROTEOMICS
ISSN
1615-9853JCR Link1615-9861JCR Link
Citation
vol. 15, no. 15, pp. 2669 - 2677
Keywords
Animal proteomicsFetal programmingObesitySecrenin 1Ubiquitin carboxy-terminal hydrolase L1
Publisher
WILEY-BLACKWELL
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
The objectives of this study were to analyze fetal programming in rat brain using proteomic analysis and to identify fetal programming-related obesity markers. Sprague-Dawley rats were divided into four feeding groups: (i) the Ad Libitum (AdLib)/AdLib group was given a normal diet during pregnancy and the lactation period; (ii) the AdLib/maternal food restriction group (FR) was subjected to 50% FR during the lactation period; (iii) the FR/AdLib group was subjected to 50% FR during pregnancy; and (iv) the FR/FR group was subjected to 50% FR during pregnancy and the lactation period. Offspring from each group were sacrificed at 3 weeks of age and whole brains were dissected. To obtain a maximum number of protein markers related to obesity, 2DE and Pathway Studio bioinformatics analysis were performed. The identities of the markers among the selected and candidate proteins were confirmed by Western blotting and immunohistochemistry. Proteomic and bioinformatics analyses revealed that expression of ubiquitin carboxy-terminal hydrolase L1 (UCHL1) and Secernin 1 (SCRN1) were significantly different in the FR/AdLib group compared with the AdLib/AdLib group for both male and female offspring. These findings suggest that UCHL1 and SCRN1 may be used as fetal programming-related obesity markers.
DOI
10.1002/pmic.201400359
Appears in Collections:
의과대학 > 의학과 > Journal papers
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