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In vitro and in vivo anti-inflammatory activity of Phyllanthus acidus methanolic extract

Title
In vitro and in vivo anti-inflammatory activity of Phyllanthus acidus methanolic extract
Authors
Hossen, Muhammad JahangirJeon, Sung HoKim, Seung CheolKim, Ji HyeJeong, DeokSung, Nak YoonYang, SungjaeBaek, Kwang-SooKim, Jun HoYoon, Deok HyoSong, Won O.Yoon, Kee DongCho, Sang-HoLee, SukchanKim, Jong-HoonCho, Jae Youl
Ewha Authors
김승철
SCOPUS Author ID
김승철scopus
Issue Date
2015
Journal Title
JOURNAL OF ETHNOPHARMACOLOGY
ISSN
0378-8741JCR Link
Citation
vol. 168, pp. 217 - 228
Keywords
Phyllanthus acidusPhyllanthaceae anti-inflammatory effect proteinTyrosine kinaseprostaglandin E-2Nuclear factor-kappa B
Publisher
ELSEVIER IRELAND LTD
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
Ethnopharmacological relevance: Phyllanthus acidus (L.) Skeels (Phyllanthaceae) has traditionally been used to treat gastric trouble, rheumatism, bronchitis, asthma, respiratory disorders, and hepatitis. Despite this widespread use, the pharmacological activities of this plant and their molecular mechanisms are poorly understood. Therefore, we evaluated the immunopharmacological activities of the methanolic extract of the aerial parts of this plant (Pa-ME) and validated its pharmacological targets. Materials and methods: Lipopolysaccharide (LPS)-treated macrophages, an HCl/EtOH-induced gastritis model, and an acetic acid-injected capillary permeability mouse model were employed to evaluate the anti-inflammatory activity of Pa-ME. Potentially active anti-inflammatory components of this extract were identified by HPLC. The molecular mechanisms of the anti-inflammatory activity were studied by kinase assays, reporter gene assays, immunoprecipitation analysis, and overexpression of target enzymes. Results: Pa-ME suppressed the production of nitric oxide (NO) and prostaglandin E-2 (PGE(2)) and prevented morphological changes in LPS-treated RAW264.7 cells. Moreover, both HCl/EtOH-induced gastric damage and acetic acid-triggered vascular permeability were restored by orally administered Pa-ME. Furthermore, this extract downregulated the expression of inducible NO synthase (iNOS) and cyclooxygenase (COX)-2 and reduced the nuclear levels of NF-kappa B. Signalling events upstream of NF-kappa B translocation, such as phosphorylation of Src and Syk and formation of Src/Syk signalling complexes, were also inhibited by Pa-ME. The enzyrintic activities of Src and Syk were also suppressed by Pa-ME. Moreover, Src-induced and Syk-induced luciferase activity and p85/Alct phosphorylation were also inhibited by Pa-ME. Of the identified flavonoids, kaempferol and quercetin were revealed as partially active anti-inflammatory components in Pa-ME. Conclusion: Pa-ME exerts anti-inflammatory activity in vitro and in vivo by suppressing Src, Syk, and their downstream transcription factor, NF-kappa B. (C) 2015 Elsevier Ireland Ltd. All rights reserved.
DOI
10.1016/j.jep.2015.03.043
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의학전문대학원 > 의학과 > Journal papers
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