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AP-1-Targeting Anti-Inflammatory Activity of the Methanolic Extract of Persicaria chinensis

Title
AP-1-Targeting Anti-Inflammatory Activity of the Methanolic Extract of Persicaria chinensis
Authors
Hossen, Muhammad JahangirKim, Seung CheolSon, Young-JinBaek, Kwang-SooKim, EunjiYang, Woo SeokJeong, DeokPark, Jae GwangKim, Han GyungChung, Woo-JaeYoon, KeejungRyou, ChongsukLee, Sang YeolKim, Jong-HoonCho, Jae Youl
Ewha Authors
김승철
SCOPUS Author ID
김승철scopus
Issue Date
2015
Journal Title
EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE
ISSN
1741-427XJCR Link1741-4288JCR Link
Publisher
HINDAWI PUBLISHING CORPORATION
Indexed
SCIE; SCOPUS WOS scopus
Abstract
In traditional Chinese medicine, Persicaria chinensis L. has been prescribed to cure numerous inflammatory disorders. We previously analyzed the bioactivity of the methanol extract of this plant (Pc-ME) against LPS-induced NO and PGE(2) in RAW264.7 macrophages and found that it preventedHCl/EtOH-induced gastric ulcers in mice. The purpose of the current study was to explore the molecular mechanism by which Pc-ME inhibits activator protein-(AP-) 1 activation pathway and mediates its hepatoprotective activity. To investigate the putative therapeutic properties of Pc-ME against AP-1-mediated inflammation and hepatotoxicity, lipopolysaccharide-(LPS-) stimulated RAW264.7 and U937 cells, a monocyte-like human cell line, and an LPS/D-galactosamine(D-GalN-) induced acute hepatitis mouse model were employed. The expression of LPS-induced proinflammatory cytokines including interleukin-(IL-) 1 beta, IL-6, and tumor necrosis factor-alpha (TNF-alpha) was significantly diminished by Pc-ME. Moreover, Pc-ME reduced AP-1 activation and mitogen-activated protein kinase (MAPK) phosphorylation in both LPS-stimulated RAW264.7 cells and differentiated U937 cells. Additionally, we highlighted the hepatoprotective and curative effects of Pc-ME pretreated orally in a mouse model of LPS/D-GalN-intoxicated acute liver injury by demonstrating the significant reduction in elevated serum AST and ALT levels and histological damage. Therefore, these results strongly suggest that Pc-ME could function as an antihepatitis remedy suppressing MAPK/AP-1-mediated inflammatory events.
DOI
10.1155/2015/608126
Appears in Collections:
의학전문대학원 > 의학과 > Journal papers
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