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Expression Levels of Chaperones Influence Biotransformation Activity of Recombinant Escherichia Coli Expressing Micrococcus Luteus Alcohol Dehydrogenase and Pseudomonas Putida Baeyer-Villiger Monooxygenase
- Expression Levels of Chaperones Influence Biotransformation Activity of Recombinant Escherichia Coli Expressing Micrococcus Luteus Alcohol Dehydrogenase and Pseudomonas Putida Baeyer-Villiger Monooxygenase
- Baek, A-Hyong; Jeon, Eun-Yeong; Lee, Sun-Mee; Park, Jin-Byung
- Ewha Authors
- SCOPUS Author ID
- Issue Date
- Journal Title
- BIOTECHNOLOGY AND BIOENGINEERING
- 0006-3592; 1097-0290
- vol. 112, no. 5, pp. 889 - 895
- chaperones; gamma-prefoldin; thermosome; whole-cell biocatalysis; Baeyer-Villiger monooxygenase; alcohol dehydrogenase; ricinoleic acid; Escherichia coli
- SCI; SCIE; SCOPUS
- We demonstrated for the first time that the archaeal chaperones (i.e., -prefoldin and thermosome) can stabilize enzyme activity in vivo. Ricinoleic acid biotransformation activity of recombinant Escherichia coli expressing Micrococcus luteus alcohol dehydrogenase and the Pseudomonas putida KT2440 Baeyer-Villiger monooxygenase improved significantly with co-expression of -prefoldin or recombinant themosome originating from the deep-sea hyperthermophile archaea Methanocaldococcus jannaschii. Furthermore, the degree of enhanced activity was dependent on the expression levels of the chaperones. For example, whole-cell biotransformation activity was highest at 12 mu mol/g dry cells/min when -prefoldin expression level was approximately 46% of the theoretical maximum. This value was approximately two-fold greater than that in E. coli, where the -prefoldin expression level was zero or set to the theoretical maximum. Therefore, it was assumed that the expression levels of chaperones must be optimized to achieve maximum biotransformation activity in whole-cell biocatalysts. Biotechnol. Bioeng. 2015;112: 889-895. (c) 2014 Wiley Periodicals, Inc.
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