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Microbial Synthesis of Plant Oxylipins from gamma-Linolenic Acid through Designed Biotransformation Pathways

Title
Microbial Synthesis of Plant Oxylipins from gamma-Linolenic Acid through Designed Biotransformation Pathways
Authors
Kim, Sae-UmKim, Kyoung-RokKim, Ji-WonKim, SoominKwon, Yong-UkOh, Deok-KunPark, Jin-Byung
Ewha Authors
박진병권용억
SCOPUS Author ID
박진병scopus; 권용억scopus
Issue Date
2015
Journal Title
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
ISSN
0021-8561JCR Link1520-5118JCR Link
Citation
vol. 63, no. 10, pp. 2773 - 2781
Keywords
plant oxylipinsgamma-linolenic acidbiotransformationwhole-cell biocatalysisEscherichia coli
Publisher
AMER CHEMICAL SOC
Indexed
SCI; SCIE; SCOPUS WOS
Abstract
Secondary metabolites of plants are often difficult to synthesize in high yields because of the large complexity of the biosynthetic pathways and challenges encountered in the functional expression of the required biosynthetic enzymes in microbial cells. In this study, the biosynthesis of plant oxylipins-a family of oxygenated unsaturated carboxylic acids-was explored to enable a high-yield production through a designed microbial synthetic system harboring a set of microbial enzymes (i.e., fatty acid double-bond hydratases, alcohol dehydrogenases, Baeyer-Villiger monooxygenases, and esterases) to produce a variety of unsaturated carboxylic acids from ?-linolenic acid. The whole cell system of the recombinant Escherichia coli efficiently produced (6Z,9Z)-12-hydroxydodeca-6,9-dienoic acid (<bold>7</bold>), (Z)-9-hydroxynon-6-enoic acid (<bold>15</bold>), (Z)-dec-4-enedioic acid (<bold>17</bold>), and (6Z,9Z)-13-hydroxyoctadeca-6,9-dienoic acid (<bold>2</bold>). This study demonstrated that various secondary metabolites of plants can be produced by implementing artificial biosynthetic pathways into whole-cell biocatalysis.
DOI
10.1021/jf5058843
Appears in Collections:
엘텍공과대학 > 식품공학전공 > Journal papers
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