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Microbial Synthesis of Plant Oxylipins from gamma-Linolenic Acid through Designed Biotransformation Pathways
- Microbial Synthesis of Plant Oxylipins from gamma-Linolenic Acid through Designed Biotransformation Pathways
- Kim, Sae-Um; Kim, Kyoung-Rok; Kim, Ji-Won; Kim, Soomin; Kwon, Yong-Uk; Oh, Deok-Kun; Park, Jin-Byung
- Ewha Authors
- 박진병; 권용억
- SCOPUS Author ID
- 박진병; 권용억
- Issue Date
- Journal Title
- JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
- 0021-8561; 1520-5118
- vol. 63, no. 10, pp. 2773 - 2781
- plant oxylipins; gamma-linolenic acid; biotransformation; whole-cell biocatalysis; Escherichia coli
- AMER CHEMICAL SOC
- SCI; SCIE; SCOPUS
- Secondary metabolites of plants are often difficult to synthesize in high yields because of the large complexity of the biosynthetic pathways and challenges encountered in the functional expression of the required biosynthetic enzymes in microbial cells. In this study, the biosynthesis of plant oxylipins-a family of oxygenated unsaturated carboxylic acids-was explored to enable a high-yield production through a designed microbial synthetic system harboring a set of microbial enzymes (i.e., fatty acid double-bond hydratases, alcohol dehydrogenases, Baeyer-Villiger monooxygenases, and esterases) to produce a variety of unsaturated carboxylic acids from ?-linolenic acid. The whole cell system of the recombinant Escherichia coli efficiently produced (6Z,9Z)-12-hydroxydodeca-6,9-dienoic acid (<bold>7</bold>), (Z)-9-hydroxynon-6-enoic acid (<bold>15</bold>), (Z)-dec-4-enedioic acid (<bold>17</bold>), and (6Z,9Z)-13-hydroxyoctadeca-6,9-dienoic acid (<bold>2</bold>). This study demonstrated that various secondary metabolites of plants can be produced by implementing artificial biosynthetic pathways into whole-cell biocatalysis.
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