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A fibrin-supported myocardial organ culture for isolation of cardiac stem cells via the recapitulation of cardiac homeostasis

Title
A fibrin-supported myocardial organ culture for isolation of cardiac stem cells via the recapitulation of cardiac homeostasis
Authors
Kim, Jong-TaeChung, Hye JinSeo, Ji-YeonYang, Young-IiChoi, Min-YoungKim, Hyeong-InYang, Tae-HyunLee, Won-JinYoun, Young ChulKim, Hye JungKim, Yeon MeeLee, HyukjinJang, Yang-SooLee, Seung-Jin
Ewha Authors
이승진이혁진정혜진
SCOPUS Author ID
이승진scopus; 이혁진scopus; 정혜진scopus
Issue Date
2015
Journal Title
BIOMATERIALS
ISSN
0142-9612JCR Link1878-5905JCR Link
Citation
vol. 48, pp. 66 - 83
Keywords
Organ cultureCardiac homeostasisCardiac stem cellsFibrinMyocardial infarctionStem cell niche
Publisher
ELSEVIER SCI LTD
Indexed
SCI; SCIE; SCOPUS WOS
Abstract
There is great interest in the development of cardiac stem cells (CSCs) cell-based therapeutics; thus, clinical translation requires an efficient method for attaining therapeutic quantities of these cells. Furthermore, an in vitro model to investigate the mechanisms regulating the cardiac homeostasis is crucial. We sought to develop a simple myocardial culture method for enabling both the recapitulation of myocardial homeostasis and the simultaneous isolation of CSCs. The intact myocardial fragments were encapsulated 3-dimensionally into the fibrin and cultured under dynamic conditions. The fibrin provided secure physical support and substratum to the myocardium, which mediated integrin-mediated cell signaling that allowed in situ renewal, outgrowth and cardiomyogenic differentiation of CSCs, mimicking myocardial homeostasis. Since our culture maintained the myocardial CSCs niches, it was possible to define the identity of in vitro renewed CSCs that situated in the interstitium between cardiomyocytes and microvessels. Lastly, the use of matrix-restricted fibrinolysis enabled the selective isolation of outgrown CSCs that retained the clonogenicity, long-term growth competency and cardiovascular commitment potential. Collectively, this myocardial culture might be used as an alternative tool for studying cardiac biology and developing cell-based therapeutics. (C) 2015 Elsevier Ltd. All rights reserved.
DOI
10.1016/j.biomaterials.2015.01.041
Appears in Collections:
약학대학 > 약학과 > Journal papers
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