View : 27 Download: 4

Tonsil-derived Mesenchymal Stem Cells Ameliorate CCl4-induced Liver Fibrosis in Mice via Autophagy Activation

Title
Tonsil-derived Mesenchymal Stem Cells Ameliorate CCl4-induced Liver Fibrosis in Mice via Autophagy Activation
Authors
Park, MinhwaKim, Yu-HeeWoo, So-YounLee, Hye JinYu, YeonsilKim, Han SuPark, Yoon ShinJo, InhoPark, Joo-WonJung, Sung-ChulLee, HyukjinJeong, ByeongmoonRyu, Kyung-Ha
Ewha Authors
유경하정병문우소연김한수정성철이혜진조인호박윤신박주원이혁진유연실김유희
SCOPUS Author ID
유경하scopus; 정병문scopus; 우소연scopus; 김한수scopus; 정성철scopus; 이혜진scopus; 조인호scopus; 박윤신scopusscopus; 박주원scopus; 이혁진scopus; 유연실scopus; 김유희scopus
Issue Date
2015
Journal Title
SCIENTIFIC REPORTS
ISSN
2045-2322JCR Link
Citation
vol. 5
Publisher
NATURE PUBLISHING GROUP
Indexed
SCI; SCIE; SCOPUS WOS
Abstract
Liver transplantation is the treatment of choice for chronic liver failure, although it is complicated by donor shortage, surgery-related complications, and immunological rejection. Cell transplantation is an alternative, minimally invasive treatment option with potentially fewer complications. We used human palatine tonsil as a novel source of mesenchymal stem cells (T-MSCs) and examined their ability to differentiate into hepatocyte-like cells in vivo and in vitro. Carbon tetrachloride (CCl4) mouse model was used to investigate the ability of T-MSCs to home to the site of liver injury. T-MSCs were only detected in the damaged liver, suggesting that they are disease-responsive. Differentiation of T-MSCs into hepatocyte-like cells was confirmed in vitro as determined by expression of hepatocyte markers. Next, we showed resolution of liver fibrosis by T-MSCs via reduction of TGF-beta expression and collagen deposition in the liver. We hypothesized that autophagy activation was a possible mechanism for T-MSC-mediated liver recovery. In this report, we demonstrate for the first time that T-MSCs can differentiate into hepatocyte-like cells and ameliorate liver fibrosis via autophagy activation and down-regulation of TGF-beta. These findings suggest that T-MSCs could be used as a novel source for stem cell therapy targeting liver diseases.
DOI
10.1038/srep08616
Appears in Collections:
의학전문대학원 > 의학과 > Journal papers
Files in This Item:
001.pdf(1.11 MB)Download
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE