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Extracellular signal-regulated kinase1/2-dependent changes in tight junctions after ischemic preconditioning contributes to tolerance induction after ischemic stroke
- Title
- Extracellular signal-regulated kinase1/2-dependent changes in tight junctions after ischemic preconditioning contributes to tolerance induction after ischemic stroke
- Authors
- Shin, Jin A.; Kim, Yul A.; Jeong, Sae Im; Lee, Kyung-Eun; Kim, Hee-Sun; Park, Eun-Mi
- Ewha Authors
- 이경은; 김희선; 박은미
- SCOPUS Author ID
- 이경은; 김희선; 박은미
- Issue Date
- 2015
- Journal Title
- BRAIN STRUCTURE & FUNCTION
- ISSN
- 1863-2653
1863-2661
- Citation
- BRAIN STRUCTURE & FUNCTION vol. 220, no. 1, pp. 13 - 26
- Keywords
- Blood-brain barrier; ERK1/2; Ischemic preconditioning; Ischemic stroke; Tight junction proteins; U0126
- Publisher
- SPRINGER HEIDELBERG
- Indexed
- SCI; SCIE; SCOPUS
- Document Type
- Article
- Abstract
- Less disruption of the blood-brain barrier (BBB) after severe ischemic stroke is one of the beneficial outcomes of ischemic preconditioning (IP). However, the effect of IP on tight junctions (TJs), which regulate paracellular permeability of the BBB, is not well understood. In the present study, we examined IP-induced changes in TJs before and after middle cerebral artery occlusion (MCAO) in mice, and the association between changes in TJs and tolerance to a subsequent insult. After IP, we found decreased levels of transmembrane TJ proteins occludin and claudin-5, and widened gaps of TJs with perivascular swelling at the ultrastructural level in the brain. An inflammatory response was also observed. These changes were reversed by inhibition of extracellular signal-regulated kinase1/2 (ERK1/2) via the specific ERK1/2 inhibitor U0126. After MCAO, reduced brain edema and inflammatory responses were associated with altered levels of angiogenic factors and cytokines in preconditioned brains. Pretreatment with U0126 reversed the neuroprotective effects of IP against MCAO. These findings suggest that ERK1/2 activation has a pivotal role in IP-induced changes in TJs and inflammatory response, which serve to protect against BBB breakdown and inflammation after ischemic stroke.
- DOI
- 10.1007/s00429-013-0632-5
- Appears in Collections:
- 의과대학 > 의학과 > Journal papers
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