Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 윤주영 | * |
dc.date.accessioned | 2016-08-27T04:08:08Z | - |
dc.date.available | 2016-08-27T04:08:08Z | - |
dc.date.issued | 2015 | * |
dc.identifier.issn | 0003-2700 | * |
dc.identifier.issn | 1520-6882 | * |
dc.identifier.other | OAK-12387 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/216993 | - |
dc.description.abstract | Fluorescent probe 1, the first inorganic phosphate (Pi) targeted colorimetric and fluorescent probe to detect endogenous Pi in hemichannel-closed cells, has been developed. Probe 1 undergoes a unique Pi induced hydrolytic reaction in DMSOHEPES (V/V = 9:1) buffered (0.02 M, pH 7.4) solutions that produces a colorimetric change associated with a 62 nm red-shift in the UVvis absorption maximum and up to a 780-fold enhancement in the fluorescence intensity. The mechanistic proposal that these spectroscopic changes are associated with reaction Pi with 1 to form coumarin gains support from the results of theoretical calculations and mass spectrometry studies. Observations made in fluorescence imaging studies with HeLa cells and C. elegans show that 1 can be employed to monitor Pi production in vivo caused by apyrase-catalyzed ATP hydrolysis. Moreover, probe 1 was utilized to show that apoptosis of hemichannel-closed Sf9 cells is caused by Inx3 promoted dephosphorylation of Akt (RAC serine/threonine-protein kinase), leading to an elevation of the concentration of Pi. Overall, the study has produced the first fluorescent sensor 1 for endogenous inorganic phosphate. Moreover, the utility of 1 for measuring Pi release in vitro has been demonstrated and utilized to elucidate the mechanism of Inx3 action in hemichannel-closed Sf9 cells. | * |
dc.language | English | * |
dc.publisher | AMER CHEMICAL SOC | * |
dc.title | Phosphate Ion Targeted Colorimetric and Fluorescent Probe and Its Use to Monitor Endogeneous Phosphate Ion in a Hemichannel-Closed Cell | * |
dc.type | Article | * |
dc.relation.issue | 2 | * |
dc.relation.volume | 87 | * |
dc.relation.index | SCI | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 1196 | * |
dc.relation.lastpage | 1201 | * |
dc.relation.journaltitle | ANALYTICAL CHEMISTRY | * |
dc.identifier.doi | 10.1021/ac503818p | * |
dc.identifier.wosid | WOS:000348332300051 | * |
dc.author.google | Guo, Lin E. | * |
dc.author.google | Zhang, Jun Feng | * |
dc.author.google | Liu, Xin Yi | * |
dc.author.google | Zhang, Li Mei | * |
dc.author.google | Zhang, Hong Li | * |
dc.author.google | Chen, Jian Hua | * |
dc.author.google | Xie, Xiao Guang | * |
dc.author.google | Zhou, Ying | * |
dc.author.google | Luo, Kaijun | * |
dc.author.google | Yoon, Juyoung | * |
dc.contributor.scopusid | 윤주영(7403587371) | * |
dc.date.modifydate | 20240118162450 | * |