View : 42 Download: 0

Characterization of pig sperm hyaluronidase and improvement of the digestibility of cumulus cell mass by recombinant pSPAM1 hyaluronidase in an in vitro fertilization assay

Title
Characterization of pig sperm hyaluronidase and improvement of the digestibility of cumulus cell mass by recombinant pSPAM1 hyaluronidase in an in vitro fertilization assay
Authors
Yoon, SungwonChang, Kyu-TaeCho, HongsangMoon, JisangKim, Ju-SungMin, Sung-HunKoo, Deog-BonLee, Sang-RaeKim, Sang-HyunPark, Ki-EunPark, Young IlKim, Ekyune
Ewha Authors
박영일
SCOPUS Author ID
박영일scopus
Issue Date
2014
Journal Title
ANIMAL REPRODUCTION SCIENCE
ISSN
0378-4320JCR Link1873-2232JCR Link
Citation
vol. 150, no. 3-4, pp. 107 - 114
Keywords
Cumulus-oocyte complexFertilizationPig SPAM1Sperm hyaluronidaseRecombinant proteinSperm-egg interaction
Publisher
ELSEVIER SCIENCE BV
Indexed
SCI; SCIE; SCOPUS WOS
Abstract
Although sperm hyaluronidase is thought to play an important role in mammalian fertilization, the molecular function underlying these steps remains largely unknown. In mouse models, sperm-specific SPAM1 and HYAL5 hyaluronidase are believed to function in both sperm penetration of the cumulus matrix and sperm-ZP binding. However, gene-targeting studies for SPAM1 or HYAL5 show that hyaluronidases are not essential for fertilization, despite the fact that exogenous hyaluronidase can disrupt the cumulus matrix. Therefore, to evaluate whether sperm hyaluronidase is essential for mammalian fertilization, it is necessary to generate HYAL5/SPAM1 double-knockout mice. However, generating double-knockout mice is very difficult because these two genes exist on the same chromosome. Recently, investigators have begun to employ the pig model system to study human disease due to its similarities to human anatomy and physiology. In this study, we confirmed that pig SPAM1 exists as a single copy gene on chromosome 18 and is specifically expressed in the testis. In addition, we expressed recombinant pig SPAM1 in human embryonic kidney 293 cells and showed that these enzymes possess hyaluronidase activity. We also demonstrated that a polyclonal antibody against pig sperm hyaluronidase inhibits sperm-egg interactions in an in vitro fertilization (IVF) assay. Our results suggest that pig SPAM1 may play a critical role in pig fertilization and that recombinant SPAM1 can disperse the oocyte-cumulus complex in an IVF assay. (C) 2014 Elsevier B.V. All rights reserved.
DOI
10.1016/j.anireprosci.2014.09.002
Appears in Collections:
신산업융합대학 > 융합콘텐츠학과 > Journal papers
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE