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3D Culture of Tonsil-Derived Mesenchymal Stem Cells in Poly(ethylene glycol)-Poly(L-alanine-co-L-phenyl alanine) Thermogel

Title
3D Culture of Tonsil-Derived Mesenchymal Stem Cells in Poly(ethylene glycol)-Poly(L-alanine-co-L-phenyl alanine) Thermogel
Authors
Park, Min HeeYu, YeonsilMoon, Hyo JungKo, Du YoungKim, Han SuLee, HyukjinRyu, Kyung HaJeong, Byeongmoon
Ewha Authors
유경하정병문김한수이혁진유연실
SCOPUS Author ID
유경하scopus; 정병문scopus; 김한수scopus; 이혁진scopus; 유연실scopus
Issue Date
2014
Journal Title
ADVANCED HEALTHCARE MATERIALS
ISSN
2192-2640JCR Link2192-2659JCR Link
Citation
vol. 3, no. 11, pp. 1782 - 1791
Publisher
WILEY-BLACKWELL
Indexed
SCIE; SCOPUS WOS
Abstract
Poly(ethylene glycol)-poly(L-alanine-co-L-phenyl alanine) (PEG-PAF) aqueous solutions undergo sol-to-gel transition as the temperature increases. The transition is driven by the micelle aggregation involving the partial dehydration of the PEG block and the partial increase in beta-sheet content of the PAF block. Tonsil-tissue-derived mesenchymal stem cells (TMSCs), a new stem cell resource, are encapsulated through the sol-to-gel transition of the TMSC-suspended PEG-PAF aqueous solutions. The encapsulated TMSCs are in vitro 3D cultured by using induction media supplemented with adipogenic, osteogenic, or chondrogenic factors, where the TMSCs preferentially undergo chondrogenesis with high expressions of type II collagen and sulfated glycosaminoglycan. As a feasibility study of the PEG-PAF thermogel for injectable tissue engineering, the TMSCs encapsulated in hydrogels are implanted in the subcutaneous layer of mice by injecting the TMSC suspended PEG-PAF aqueous solution. The in vivo studies also prove that TMSCs undergo chondrogenesis with high expression of the chondrogenic biomarkers. This study suggests that the TMSCs can be an excellent resource of MSCs, and the thermogelling PEG-PAF is a promising injectable tissue engineering scaffold, particularly for chondrogenic differentiation of the stem cells.
DOI
10.1002/adhm.201400140
Appears in Collections:
의학전문대학원 > 의학과 > Journal papers
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