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Development of an Albumin Copper Binding (ACuB) Assay to Detect Ischemia Modified Albumin

Title
Development of an Albumin Copper Binding (ACuB) Assay to Detect Ischemia Modified Albumin
Authors
Eom, Ji-EunLee, EunyoungJeon, Kyung-HwaSim, JeongeunSuh, MinahJhon, Gil-JaKwon, Youngjoo
Ewha Authors
전길자권영주
SCOPUS Author ID
전길자scopus; 권영주scopus
Issue Date
2014
Journal Title
ANALYTICAL SCIENCES
ISSN
0910-6340JCR Link1348-2246JCR Link
Citation
vol. 30, no. 10, pp. 985 - 990
Keywords
Albumin copper binding (ACuB) assayischemia modified albuminserum IMA levels in stroke rat models96-well volume reaction
Publisher
JAPAN SOC ANALYTICAL CHEMISTRY
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
Myocardial ischemia (MI) induces many changes in the body, including pH decrease and electrolyte imbalance. No obvious symptoms of MI appear until irreversible cellular injuries occur. Since early treatment is critical for recovery from ischemia, the development of reliable diagnostic tool is demanded to detect the early ischemic status. Ischemia modified albumin (IMA), formed by cleavage of the last two amino acids of the human serum albumin (HSA) N-terminus, has been considered so far as the most trustworthy and accurate marker for the investigation of ischemia. IMA levels are elevated in plasma within a few minutes of ischemic onset, and may last for up to 6 h. In the present study, we developed a novel assay for the examination of IMA levels to ameliorate the known albumin cobalt binding (ACB) test established previously. We observed a stronger copper ion bound to the HSA N-terminal peptide than cobalt ion by HPLC and ESI-TOF mass spectrometric analyses. The copper ion was employed with lucifer yellow (LY), a copper-specific reagent to develop a new albumin copper binding (ACuB) assay. The parameters capable of affecting the assay results were optimized, and the finally-optimized ACuB assay was validated. The result of the IMA level measurement in normal versus stroke rat serum suggests that the ACuB assay is likely to be a reliable and sensitive method for the detection of ischemic states.
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자연과학대학 > 화학·나노과학전공 > Journal papers
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