Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 방은경 | * |
dc.date.accessioned | 2016-08-27T04:08:55Z | - |
dc.date.available | 2016-08-27T04:08:55Z | - |
dc.date.issued | 2014 | * |
dc.identifier.issn | 2314-6133 | * |
dc.identifier.issn | 2314-6141 | * |
dc.identifier.other | OAK-11719 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/216876 | - |
dc.description.abstract | Biomaterials are widely used as scaffolds for tissue engineering. We have developed a strategy for bone tissue engineering that entails application of immobilized anti-BMP-2 monoclonal antibodies (mAbs) to capture endogenous BMPs in vivo and promote antibody-mediated osseous regeneration (AMOR). The purpose of the current study was to compare the efficacy of immobilization of a specific murine anti-BMP-2 mAb on three different types of biomaterials and to evaluate their suitability as scaffolds for AMOR. Anti-BMP-2 mAb or isotype control mAb was immobilized on titanium (Ti) microbeads, alginate hydrogel, and ACS. The treated biomaterials were surgically implanted in rat critical-sized calvarial defects. After 8 weeks, de novo bone formation was assessed using micro-CT and histomorphometric analyses. Results showed de novo bone regeneration with all three scaffolds with immobilized anti-BMP-2 mAb, but not isotype control mAb. Ti microbeads showed the highest volume of bone regeneration, followed by ACS. Alginate showed the lowest volume of bone. Localization of BMP-2, -4, and -7 antigens was detected on all 3 scaffolds with immobilized anti-BMP-2mAb implanted in calvarial defects. Altogether, these data suggested a potential mechanism for bone regeneration through entrapment of endogenous BMP-2, -4, and -7 proteins leading to bone formation using different types of scaffolds via AMOR. | * |
dc.language | English | * |
dc.publisher | HINDAWI PUBLISHING CORPORATION | * |
dc.title | Immobilization of Murine Anti-BMP-2 Monoclonal Antibody on Various Biomaterials for Bone Tissue Engineering | * |
dc.type | Article | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.journaltitle | BIOMED RESEARCH INTERNATIONAL | * |
dc.identifier.doi | 10.1155/2014/940860 | * |
dc.identifier.wosid | WOS:000340159400001 | * |
dc.author.google | Ansari, Sahar | * |
dc.author.google | Freire, Marcelo O. | * |
dc.author.google | Pang, Eun-Kyoung | * |
dc.author.google | Abdelhamid, Alaa I. | * |
dc.author.google | Almohaimeed, Mohammad | * |
dc.author.google | Zadeh, Homayoun H. | * |
dc.contributor.scopusid | 방은경(8058781900) | * |
dc.date.modifydate | 20231123093533 | * |