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Osteoclast activation by receptor activator of NF-kappa B ligand enhances the mobilization of hematopoietic progenitor cells from the bone marrow in acute injury

Title
Osteoclast activation by receptor activator of NF-kappa B ligand enhances the mobilization of hematopoietic progenitor cells from the bone marrow in acute injury
Authors
Cho, Kyung-AhJoo, Sun-YoungHan, Ho-SeongRyu, Kyung-HaWoo, So-Youn
Ewha Authors
한호성유경하우소연조경아
SCOPUS Author ID
유경하scopus; 우소연scopus; 조경아scopus
Issue Date
2010
Journal Title
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
ISSN
1107-3756JCR Link
Citation
vol. 26, no. 4, pp. 557 - 563
Keywords
osteoclastshematopoietic stem cellsCXCR4SDF-1and osteoblasts
Publisher
SPANDIDOS PUBL LTD
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
Osteoclasts (OCLs) are multinucleated cells that are derived from the monocyte/macrophage hematopoietic lineage in response to receptor activator of NF-kappa B ligand (RANKL) activation. They are specialized cells responsible for physiological bone resorption and as well as pathologic bone loss. In addition to their unique ability to resorb bone, OCLs also play a potential role in the mobilization of hematopoietic progenitor cells from the bone marrow (BM), particularly under various stress stimuli (e.g. hypoxia, injury or inflammation). We investigated the effect of activated OCLs on the stem cell niche and whether this leads to mobiliz-ation of hematopoietic progenitors. We induced activated OCLs from the RAW264.7 cell line through stimulation with RANKL and we quantified the levels of the stem cell niche component SDF-1 on the osteblasts and CXCR4 on the bone marrow cells (BMCs) by culturing with supernatants from activated OCLs. In addition, we exposed mice to stress by inducing liver injury with CCl(4) followed by injecting RANKL to activate OCLs and compared the effect on the mobilization of hematopoietic progenitor cells from the BM. We found that functional OCLs cleaved SDF-1 alpha in the osteoblasts and increased CXCR4 expression in the BMCs. Moreover, under stress in vivo, mobilized hematopoietic progenitor cells were significantly increased after RANKL treatment. These results suggest that OCLs might be involved in alteration of the interaction between SDF-1 and CXCR4 leading to mobilization of hematopoietic progenitor cells from the BM.
DOI
10.3892/ijmm_00000499
Appears in Collections:
의과대학 > 의학과 > Journal papers
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