Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 강덕희 | - |
dc.contributor.author | 유민아 | - |
dc.date.accessioned | 2016-08-27T02:08:06Z | - |
dc.date.available | 2016-08-27T02:08:06Z | - |
dc.date.issued | 2010 | - |
dc.identifier.issn | 0250-8095 | - |
dc.identifier.other | OAK-6173 | - |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/216420 | - |
dc.description.abstract | Background: Epithelial-mesenchymal transition (EMT) is important in the development of peritoneal fibrosis. Glucose degradation products (GDPs) may induce EMT in human peritoneal mesothelial cells (HPMCs). Methods: The effects of individual GDPs and GDPs derived from peritoneal dialysis fluid (PDF) in both HPMCs and peritoneal membranes were evaluated. EMT was assessed with alpha-smooth muscle actin (alpha-SMA) and E-cadherin. Results: In vitro, alpha-SMA protein and mRNA levels increased in the presence of the GDPs (formaldehyde, glyoxal, methylglyoxal, and 3-deoxyglucosone), and E-cadherin decreased. Changes in the EMT markers were most prominent after exposure to 3-deoxyglucosone. Changes in both alpha-SMA and E-cadherin protein levels were less with low (L)-GDP bicarbonate/lactate-buffered PDF compared to high (H)-GDP PDF. In the rat model after 8 weeks' PDF infusion, the alpha-SMA/E-cadherin mRNA ratio increased in the H-GDP group compared with the L-GDP group (p < 0.05). The peritoneum in the H-GDP group tended to be thicker (p = 0.052) and had more blood vessels than that in the L-GDP group (p < 0.05). Tissue staining for TGF-beta 1 decreased in the L-GDP group. Dual-stained cytokeratin and alpha-SMA-positive myofibroblasts in the submesothelial layer were more prominent in the H-GDP group. Conclusion: GDPs found in PDF induce EMT of HPMCs, which is associated with peritoneal fibrosis and vascularization. Conversely, L-GDP PDF reduces EMT and peritoneal fibrosis. Copyright (c) 2009 S. Karger AG, Basel | - |
dc.language | English | - |
dc.publisher | KARGER | - |
dc.subject | Epithelial-mesenchymal transition | - |
dc.subject | Glucose degradation products | - |
dc.subject | Vascularization | - |
dc.subject | Peritoneal fibrosis | - |
dc.subject | Mesothelial cells | - |
dc.title | Impact of Low Glucose Degradation Product Bicarbonate/Lactate-Buffered Dialysis Solution on the Epithelial-Mesenchymal Transition of Peritoneum | - |
dc.type | Article | - |
dc.relation.issue | 1 | - |
dc.relation.volume | 31 | - |
dc.relation.index | SCI | - |
dc.relation.index | SCIE | - |
dc.relation.index | SCOPUS | - |
dc.relation.startpage | 58 | - |
dc.relation.lastpage | 67 | - |
dc.relation.journaltitle | AMERICAN JOURNAL OF NEPHROLOGY | - |
dc.identifier.doi | 10.1159/000256658 | - |
dc.identifier.wosid | WOS:000273246300009 | - |
dc.author.google | Oh, Eun-Joo | - |
dc.author.google | Ryu, Hye-Myung | - |
dc.author.google | Choi, Soon-Youn | - |
dc.author.google | Yook, Ju-Min | - |
dc.author.google | Kim, Chan-Duck | - |
dc.author.google | Park, Sun-Hee | - |
dc.author.google | Chung, Ho-Young | - |
dc.author.google | Kim, In-San | - |
dc.author.google | Yu, Min-A | - |
dc.author.google | Kang, Duk-Hee | - |
dc.author.google | Kim, Yong-Lim | - |
dc.contributor.scopusid | 강덕희(17233695600) | - |
dc.date.modifydate | 20230210140157 | - |