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Genetic toxicity test of 8-hydroxyquinoline by ames, micronucleus, comet assays and microarray analysis

Title
Genetic toxicity test of 8-hydroxyquinoline by ames, micronucleus, comet assays and microarray analysis
Authors
Lee, Woo SunKim, Hyun JooLee, Eun MiKim, Joo HwanSuh, Soo KyungKwon, Kyung JinSheen, Yhun YongKim, Seung HeePark, Sue N.
Ewha Authors
신윤용
SCOPUS Author ID
신윤용scopus
Issue Date
2007
Journal Title
MOLECULAR & CELLULAR TOXICOLOGY
ISSN
1738-642XJCR Link
Citation
vol. 3, no. 2, pp. 90 - 97
Keywords
8-hydroxyquinolineames testCOMET assayMN assaymicroarrayS9 fraction
Publisher
KOREAN SOC TOXICOGENOMICS & TOXICOPROTEOMICS
Indexed
SCIE; SCOPUS; KCI WOS
Abstract
8-Hydroxyquinoline is used as antibacterial agent and antioxidant based on its function inducing the chelation of ferrous ion present in host resulting in production of chelated complex. This complex being transported to cell membrane of bacteria and fungi exerts antibacterial and antifungal action. In this study, we have carried out in vitro genetic toxicity tests and microarray analysis to understand the underlying mechanisms and the mode of action of toxicity of 8-hydroxyquinoline. TA1535 and TA98 cells were treated with 8-hydroxyquinoline to test its toxicity by basic genetic toxicity test, Ames and two new in vitro micronucleus and COMET assays were applied using CHO cells and L5178Y cells, respectively. In addition, microarray analysis of differentially expressed genes in L5178Y cells in response to 8-hydroxyquinoline were analyzed using Affymatrix genechip. The result of Ames test was that 8-hydroxyquinoline treatment increased the mutations in base substitution strain TA1535 and likewise, 8-hydroxyquinoline also increased mutations in frame shift TA98. 8-Hydroxyquinoline increased micronuclei in CHO cells and DNA damage in L5178Y. 8-Hydroxyquinoline resulted in positive response in all three tests showing its ability to induce not only mutation but also DNA damage. 783 Genes were initially selected as differentially expressed genes in response to 8-hydroxyquinoline by microarray analysis and 34 genes among them were over 4 times of log fold changed. These 34 genes could be candidate biomarkers of genetic toxic action of 8-hydroxyquinoline related to induction of mutation and/or induction of micronuclei and DNA damage. Further confirmation of these candidate markers related to their biological function will be useful to understand the detailed mode of action of 8-hydroxyquinoline.
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약학대학 > 약학과 > Journal papers
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