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Bacterial DNA containing methylated CpG motifs retains immunostimulatory activity in synergy with modified lipopolysaccharides

Title
Bacterial DNA containing methylated CpG motifs retains immunostimulatory activity in synergy with modified lipopolysaccharides
Authors
Cho, Yang JeAhn, Bo YoungSong, Eun SunPark, Shin AeLee, Dong HyeonKim, Doo-SikLee, Na Gyong
Ewha Authors
이동현
SCOPUS Author ID
이동현scopus
Issue Date
2007
Journal Title
MICROBIOLOGY AND IMMUNOLOGY
ISSN
0385-5600JCR Link
Citation
vol. 51, no. 2, pp. 211 - 222
Keywords
bacterial DNAmethylated CpGimmunostimulatory activity
Publisher
CENTER ACADEMIC PUBL JAPAN
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
We previously described the immunostimulatory activity of CIA07, a combination of bacterial DNA fragments and modified LPS, and demonstrated that CIA07 has antitumor activity in a mouse bladder cancer model. In this study, we investigated whether methylation of the CpG motifs on the bacterial DNA fragments affects the immunostimulatory potential of CIA07. E. coli DNA fragments were methylated with CpG methylase, and then combined with modified LPS for experiments. Our results revealed that methylated CIA07 (mCIA07) and unmethylated CIA07 were equally active in inducing cytokine secretion from human whole blood cells. In addition, both methylated DNA fragments and mCIA07 retained the ability to activate expression and nuclear translocation of NF-kappa B in RAW 264.7 cells. Finally, methylated DNA fragments and mCIA07 exhibited an antitumor activity comparable to those of their unmethylated counterparts in our mouse bladder cancer model. These data demonstrate that CpG methylation of E. coli DNA does not abrogate the immunostimulatory activity of DNA fragments or CIA07, suggesting that the synergistic activity by bacterial DNA in combination with LPS may be independent of the methylation status of CpG motifs.
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의과대학 > 의학과 > Journal papers
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