View : 20 Download: 0

CCR7 is critically important in intestinal lamina propria for migration of dendritic cells to mesenteric lymph nodes

Title
CCR7 is critically important in intestinal lamina propria for migration of dendritic cells to mesenteric lymph nodes
Authors
Jang, MHSougawa, NTanaka, THirata, THiroi, TTohya, KGuo, ZJUmemoto, EEbisuno, YYang, BGSeoh, JYLipp, MKiyono, HMiyasaka, M
Ewha Authors
서주영
SCOPUS Author ID
서주영scopus
Issue Date
2006
Journal Title
JOURNAL OF IMMUNOLOGY
ISSN
0022-1767JCR Link
Citation
vol. 176, no. 2, pp. 803 - 810
Publisher
AMER ASSOC IMMUNOLOGISTS
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
Although dendritic cells (DCs) located in the small intestinal lamina propria (LP-DCs) migrate to mesenteric lymph nodes (MLNs) constitutively, it is unclear which chemokines regulate their trafficking to NILNs. In this study we report that LP-DCs in unperturbed mice require CCR7 to migrate to MLNs. In vitro, LP-DCs expressing CCR7 migrated toward CCL21, although the LP-DCs appeared morphologically and phenotypically immature. In NILNs, DCs bearing the unique LP-DC phenotype (CD11c(high)CD8 alpha(int)CD11b(low)alpha(L)(low)beta(7) and CD11c(high)CD8 alpha(-)CD11b(high) alpha(L)(low)beta(7)(high)) were abundant in wild-type mice, but were markedly fewer in CCL19-, CCL21-Ser-deficient plt/plt mice and were almost absent in CCR7-deficient mice, indicating the critical importance of CCR7 in LP-DC trafficking to MLNs. Interestingly, CCR7(+) DCs in MLNs with the unique LP-DC phenotype had numerous vacuoles containing cellular debris in the cytoplasm, although MLN-DCs themselves were poorly phagocytic, suggesting that the debris was derived from the LP, where the LP-DCs ingested apoptotic intestinal epithelial cells (IECs). Consistent with this, LP-DCs ingested IECs vigorously in vitro. By presenting IEC-associated Ag, the LP-DCs also induce T cells to produce IL-4 and IL-10. Collectively, these results strongly suggest that LP-DCs with unique immunomodulatory activities migrate to NILNs in a CCR7-dependent manner to engage in the presentation of IEC-associated Ags acquired in the LP.
Appears in Collections:
의학전문대학원 > 의학과 > Journal papers
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE