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Different responses by cultured aortic and venous smooth muscle cells to gamma radiation

Title
Different responses by cultured aortic and venous smooth muscle cells to gamma radiation
Authors
Kim, SJMasaki, TRowley, RLeypoldt, JKMohammad, SFCheung, AK
Ewha Authors
김승정
SCOPUS Author ID
김승정scopus
Issue Date
2005
Journal Title
KIDNEY INTERNATIONAL
ISSN
0085-2538JCR Link
Citation
vol. 68, no. 1, pp. 371 - 377
Keywords
hemodialysisvascular accesssmooth muscle cellradiation
Publisher
BLACKWELL PUBLISHING INC
Indexed
SCI; SCIE; SCOPUS WOS
Abstract
Background. Stenosis of hemodialysis arteriovenous grafts is usually focal and caused by the proliferation of vascular smooth muscle cells (SMCs). External radiation of the graft is a potential strategy to prevent stenosis; however, the relative responsiveness of arterial and venous SMCs to radiation is unknown. Methods. Human aortic and saphenous vein SMCs were cultured in a medium containing growth factors and serum and treated with 0 to 50 Gy in a gamma irradiator. At 2 to 20 days post-irradiation, cell counting, methylthiazoletetrazolium dye reduction, [H-3]-thymidine uptake, and bromodeoxyuridine (BrdU) incorporation assays were performed. Results. All assays showed that 1 to 50 Gy inhibited the proliferation of both aortic and venous SMCs in a dose-dependent manner. Importantly, venous cells were less susceptible to radiation in all assays, compared to aortic cells. At day 10, 1 to 50 Gy of radiation inhibited the increase in the number of aortic cells by 24% to 66% and venous cells by 8% to 25% (P < 0.01) (aortic vs. venous). The differences between aortic and venous cells varied among different assays and were most pronounced in the BrdU assay. Conclusion. Inasmuch as myointimal hyperplasia occurs at both arterial and venous anastomoses, future strategies using radiation to prevent hemodialysis vascular access stenosis should take these differences into consideration.
DOI
10.1111/j.1523-1755.2005.00407.x
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의학전문대학원 > 의학과 > Journal papers
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